Mandal Preeti, Zhao Jiajia, Sah Sanjay Kumar, Huang Yuting, Liu Jiarong
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China.
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China.
J Endod. 2014 Aug;40(8):1156-9. doi: 10.1016/j.joen.2014.01.025. Epub 2014 Mar 20.
The primary aim of this study was to evaluate the cytotoxic effects of GuttaFlow 2 (Coltène Whaledent, GmBH+Co KG, Langenau, Switzerland) on human gingival fibroblasts (HGFs).
Samples of the test materials GuttaFlow 2, mineral trioxide aggregate (MTA), AH Plus (Dentsply DeTrey, Konstanz, Germany), and RealSeal sealer (SybronEndo, Orange, CA) were fabricated in cylindrical nonreactive plastic tubes of 3-mm diameter and 2-mm height. Extracts of freshly mixed and set samples were prepared using the ratio of 0.5 cm(2)/mL, 1 cm(2)/mL, and 1.5 cm(2)/mL according to ISO 10993 series. The extracts were incubated with HGF cells for 24 and 72 hours. A cell counting kit-8 assay (Dojindo, Kumamoto, Japan) assay was used to examine cytotoxicity. The results were analyzed with the independent t test and 1-way analysis of variance test (P < .05).
At all experimental conditions, the extracts of freshly mixed GuttaFlow 2 were nontoxic, whereas the extracts of freshly mixed and set AH Plus and RealSeal sealers were toxic to HGF cells (P < .05). The extracts of set GuttaFlow 2 were toxic at 72 hours (P < .05) and nontoxic at 24 hours. The extracts of freshly mixed MTA were nontoxic at both time points. For the extracts of set MTA, 1.5 cm(2)/mL was toxic at 72 hours and 1.5 cm(2)/mL and 1 cm(2)/mL were toxic at 24 hours (P < .05).
Both GuttaFlow 2 and MTA evoked a less toxic response to HGF cells than AH Plus and RealSeal sealer.
本研究的主要目的是评估GuttaFlow 2（科尔tene惠尔登特公司，瑞士朗根瑙）对人牙龈成纤维细胞（HGFs）的细胞毒性作用。
将测试材料GuttaFlow 2、三氧化矿物凝聚体（MTA）、AH Plus（德国登士柏德特雷公司，康斯坦茨）和RealSeal封闭剂（美国赛邦恩多公司，加利福尼亚州奥兰治）制成直径3毫米、高2毫米的圆柱形非反应性塑料管样本。根据ISO 10993系列标准，以0.5平方厘米/毫升、1平方厘米/毫升和1.5平方厘米/毫升的比例制备新鲜混合和凝固样本的提取物。将提取物与HGF细胞孵育24小时和72小时。使用细胞计数试剂盒-8检测法（日本熊本同仁化学研究所）检测细胞毒性。结果采用独立t检验和单因素方差分析进行分析（P <.05）。
在所有实验条件下，新鲜混合的GuttaFlow 2提取物无毒，而新鲜混合和凝固的AH Plus及RealSeal封闭剂提取物对HGF细胞有毒（P <.05）。凝固的GuttaFlow 2提取物在72小时时有毒（P <.05），在24小时时无毒。新鲜混合的MTA提取物在两个时间点均无毒。对于凝固的MTA提取物，1.5平方厘米/毫升在72小时时有毒，1.5平方厘米/毫升和1平方厘米/毫升在24小时时有毒（P <.05）。
与AH Plus和RealSeal封闭剂相比，GuttaFlow 2和MTA对HGF细胞的毒性反应较小。
