Wang Li-Qiong, Cai Ling-Yan, Ma Yu-Long
State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia University Helanshan Rd. 539 Yinchuan 750021 China
RSC Adv. 2020 Oct 19;10(63):38409-38415. doi: 10.1039/d0ra04965d. eCollection 2020 Oct 15.
The inhibitory effects of the main inhibitors formed during acid pretreatment of corn stalk were studied through ethanol fermentations of model substrates and hydrolysates from corn stalk by alcohol yeast. Experimental results showed that the tested inhibitors had no significant effect on ethanol fermentations when they were added separately at a concentration according to analysis results from hydrolysate of corn stalk. However, when they were added as a mixture, the inhibitory effects became obvious. With the increase of concentration, there was a delay in ethanol productivity. But complete inhibition was observed at 5.0 g L furfural, 10.0 g L acetic acid, 7.0 g L ferulic acid, and 7.0 g L -coumaric acid, respectively. The inhibitory effect decreased in the order: furfural > acetic acid > ferulic acid > -coumaric acid > HMF. These results suggest that a high concentration of inhibitor has a strong negative influence on ethanol fermentation, but the inhibiting abilities of various inhibitors are different.
通过酒精酵母对玉米秸秆模型底物和水解产物进行乙醇发酵，研究了玉米秸秆酸预处理过程中形成的主要抑制剂的抑制作用。实验结果表明，当按照玉米秸秆水解产物的分析结果以一定浓度单独添加测试抑制剂时，它们对乙醇发酵没有显著影响。然而，当它们作为混合物添加时，抑制作用变得明显。随着浓度的增加，乙醇生产率出现延迟。但分别在糠醛浓度为5.0 g/L、乙酸浓度为10.0 g/L、阿魏酸浓度为7.0 g/L和对香豆酸浓度为7.0 g/L时观察到完全抑制。抑制作用的顺序为：糠醛＞乙酸＞阿魏酸＞对香豆酸＞羟甲基糠醛。这些结果表明，高浓度的抑制剂对乙醇发酵有很强的负面影响，但各种抑制剂的抑制能力不同。
