Unitat de Biocatàlisi Aplicada associada al IQAC (CSIC-UAB), Escola d'Enginyeria, UAB, 08193 Bellaterra, Spain.
Bioresour Technol. 2010 Apr;101(7):2259-66. doi: 10.1016/j.biortech.2009.11.089. Epub 2009 Dec 23.
Growth and activity of the white-rot fungus Trametes versicolor on sewage sludge were assessed in bioslurry and solid-phase systems. Bioslurry cultures with different loads of sludge (10%, 25% and 38%, w/v) were performed. A lag phase of at least 2 d appeared in the 25 and 38%-cultures, however, the total fungal biomass was higher for the latter and lower for the 10%-culture after 30 d, as revealed by ergosterol determination. Detectable laccase activity levels were found in the 10 and 25%-cultures (up to 1308 and 2588 AUL(-1), respectively) while it was negligible in the 38%-culture. Important levels of ergosterol and laccase were obtained over a 60 d period in sludge solid-phase cultures amended with different concentrations of wheat straw pellets as lignocellulosic bulking material. Degradation experiments in 25%-bioslurry cultures spiked with naproxene (NAP, analgesic) and carbamazepine (CBZ, antiepileptic) showed depletion of around 47% and 57% within 24h, respectively. Complete depletion of NAP and around 48% for CBZ were achieved within 72 h in sludge solid cultures with 38% bulking material. CBZ degradation is especially remarkable due to its high persistence in wastewater treatment plants. Results showed that T. versicolor may be an interesting bioremediation agent for elimination of emerging pollutants in sewage sludge.
研究了白腐真菌彩绒革盖菌在生物泥浆和固相中对污水污泥的生长和活性。进行了不同污泥负荷（10%、25%和 38%，w/v）的生物泥浆培养。在 25%和 38%的培养物中出现了至少 2 天的迟滞期，然而，30 天后，真菌总生物量对于后者更高，对于 10%的培养物更低，这是通过麦角固醇测定揭示的。在 10%和 25%的培养物中检测到可检测的漆酶活性水平（分别高达 1308 和 2588 AUL(-1)），而在 38%的培养物中则可以忽略不计。在添加不同浓度的麦秸颗粒作为木质纤维素填充材料的污泥固相反硝化培养物中，在 60 天的时间内获得了重要水平的麦角固醇和漆酶。在添加萘普生（NAP，镇痛药）和卡马西平（CBZ，抗癫痫药）的 25%生物泥浆培养物中进行的降解实验表明，分别在 24 小时内消耗了约 47%和 57%。在含有 38%填充材料的污泥固相反硝化培养物中，在 72 小时内完全耗尽了 NAP 和大约 48%的 CBZ。由于 CBZ 在废水处理厂中的高持久性，CBZ 的降解尤为显著。结果表明，彩绒革盖菌可能是一种用于消除污水污泥中新兴污染物的有前途的生物修复剂。
