Department of Reproductive Medicine and Gynecology, University Medical Center Utrecht, Utrecht, the Netherlands.
Fertil Steril. 2010 Oct;94(5):1764-8. doi: 10.1016/j.fertnstert.2009.10.044. Epub 2009 Dec 11.
To elucidate the impact of ovarian stimulation on the intrauterine milieu represented by the cytokine, chemokine, and growth factor profile in endometrial secretions aspirated before embryo transfer.
Prospective cohort study.
Fertility center in tertiary referral university hospital.
PATIENT(S): Forty-two patients undergoing ovarian stimulation with GnRH analogues were recruited. They participated in both a natural and an ovarian-stimulated cycle for within patient comparisons.
INTERVENTION(S): Endometrial secretion aspiration was performed immediately before embryo transfer.
MAIN OUTCOME MEASURE(S): The concentrations of 17 mediators known to be involved in human embryo implantation were assessed by multiplex immunoassay.
RESULT(S): After correction for multiple testing, significantly higher concentrations of interleukin (IL)-1β, IL-5, IL-10, IL-12, IL-17, tumor necrosis factor (TNF)-α, heparin-binding epidermal growth factor (HbEGF), eotaxin, and dickkopf homologue-1 were present in endometrial secretions obtained in stimulated compared with natural cycles.
CONCLUSION(S): Endometrial secretion analysis provides a novel means of investigating the effect of ovarian stimulation on the intrauterine milieu. The in vivo milieu encountered by the embryo after transfer is significantly altered by ovarian stimulation.
阐明卵巢刺激对胚胎移植前抽吸的子宫内膜分泌液中细胞因子、趋化因子和生长因子谱所代表的宫内环境的影响。
前瞻性队列研究。
三级转诊大学医院的生育中心。
招募了 42 名接受 GnRH 类似物卵巢刺激的患者。他们参加了自然周期和卵巢刺激周期，以便进行患者内比较。
胚胎移植前立即进行子宫内膜分泌液抽吸。
通过多重免疫分析评估已知参与人类胚胎着床的 17 种介质的浓度。
经多次检验校正后，与自然周期相比，刺激周期中子宫内膜分泌液中白细胞介素（IL）-1β、IL-5、IL-10、IL-12、IL-17、肿瘤坏死因子（TNF）-α、肝素结合表皮生长因子（HbEGF）、嗜酸性粒细胞趋化因子、骨形成蛋白同源物-1 的浓度明显升高。
子宫内膜分泌分析提供了一种研究卵巢刺激对宫内环境影响的新方法。胚胎移植后胚胎所遇到的体内环境因卵巢刺激而发生显著改变。
