Lottner Susanne, Shehata Mostafa, Hickel Reinhard, Reichl Franx-Xaver, Durner Jürgen
Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestr. 70, 80336 Munich, Germany.
Dent Mater. 2013 Sep;29(9):991-8. doi: 10.1016/j.dental.2013.07.005. Epub 2013 Jul 30.
(Co)monomers from dental resin composites have cytotoxic and genotoxic potential. In previous studies it has been demonstrated that antioxidants can decrease the cytotoxicity of various dental (co)monomers. In this study the effects of the antioxidants N-acetylcysteine (ACC) and ascorbic acid (Asc) on the number of DNA double-strand breaks (DSBs) in human gingiva fibroblasts (HGFs) were tested.
HGF was incubated with the (co)monomers bisphenol-A-glycidyl methacrylate (BisGMA), urethandimethacrylate (UDMA), ethylene glycol dimethacrylate (EGDMA) or 1,3-glyceroldimethacrylate (GDMA) with and without addition of antioxidants ACC and Asc. DNA-DSBs were determined using the γ-H2AX assay.
Asc induced at 500μM significant more DNA-DSBs in HGFs compared with controls (4.92 (1.28) vs. 1.62 (0.67); foci/cell mean (standard deviation), n=3). Most DNA-DSBs were found after incubation of HGFs with 90μM BisGMA (4.05 (0.56)) and 2720μM EGDMA (5.36 (1.59)). The addition of 100μM Asc or 500μM ACC leaded to a statistical significant reduction of DNA-DSBs in HGFs for all tested (co)monomers. After incubation of HGFs with 2720μM EGDMA and 500μM ACC the foci/cell decrease from 5.36 (1.59) to 1.9 (1.17) (controls: 1.12 (0.24)). After incubation of HGFs with 90μM BisGMA and 100μM Asc the foci/cell decrease from 4.05 (0.56) to 1.96 (0.59) (controls: 1.12 (0.24)).
All tested (co)monomers can induce DNA-DSBs but addition of antioxidants (Asc or ACC) leads to reduction of DNA-DSBs.
牙科树脂复合材料的（共）单体具有细胞毒性和基因毒性潜力。在先前的研究中已证明抗氧化剂可降低各种牙科（共）单体的细胞毒性。在本研究中，测试了抗氧化剂N - 乙酰半胱氨酸（ACC）和抗坏血酸（Asc）对人牙龈成纤维细胞（HGFs）中DNA双链断裂（DSB）数量的影响。
将HGF与双酚A - 甲基丙烯酸缩水甘油酯（BisGMA）、二甲基丙烯酸脲（UDMA）、乙二醇二甲基丙烯酸酯（EGDMA）或1,3 - 甘油二甲基丙烯酸酯（GDMA）等（共）单体一起孵育，同时添加或不添加抗氧化剂ACC和Asc。使用γ - H2AX检测法测定DNA - DSB。
与对照组相比，500μM的Asc在HGFs中诱导产生的DNA - DSB显著更多（4.92（1.28）对1.62（0.67）；病灶/细胞平均值（标准差），n = 3）。在HGFs与90μM BisGMA（4.05（0.56））和2720μM EGDMA（5.36（1.59））孵育后发现大多数DNA - DSB。添加100μM Asc或500μM ACC可使所有测试的（共）单体在HGFs中的DNA - DSB在统计学上显著减少。在HGFs与2720μM EGDMA和500μM ACC孵育后，病灶/细胞从5.36（1.59）降至1.9（1.17）（对照组：1.12（0.24））。在HGFs与90μM BisGMA和100μM Asc孵育后，病灶/细胞从4.05（0.56）降至1.96（0.59）（对照组：1.12（0.24））。
所有测试的（共）单体均可诱导DNA - DSB，但添加抗氧化剂（Asc或ACC）可导致DNA - DSB减少。
