不同共单体从纳米复合牙科复合材料中洗脱后诱导的细胞毒性和活性氧物质的产生。
Cytotoxicity and reactive oxygen species production induced by different co-monomer eluted from nanohybrid dental composites.
机构信息
Department of Dental Biomaterials Science, School of Dentistry and Dental Research Institute, Seoul National University, 101 Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea.
Department of Stomatology, Yanbian University and Affiliated Hospital of Yanbian University, Yanji, 133000, China.
出版信息
BMC Oral Health. 2023 Jan 30;23(1):55. doi: 10.1186/s12903-023-02710-y.
BACKGROUND
Safety issues for dental restorative composites are critical to material selection, but, limited information is available to dental practitioners. This study aimed to compare the chemical and biological characteristics of three nanohybrid dental composites by assessing filler particle analysis, monomer degree of conversion (DC), the composition of eluates, and cytotoxicity and reactive oxygen species (ROS) production in fibroblasts.
METHODS
Three nanohybrid composites (TN, Tetric N-Ceram; CX, Ceram X Sphere Tec One; and DN, DenFil NX) were used. The size distribution and morphology of the filler particles were analysed using scanning electron microscopy (n = 5). The DC was measured via micro-Raman spectroscopy (n = 5). For the component analysis, methanol eluates from the light-polymerised composites were evaluated by gas chromatography/mass spectrometry (n = 3). The eluates were prepared from the polymerised composites after 24 h in a cell culture medium. A live/dead assay (n = 9) and Water-Soluble Tetrazolium-1 assay (n = 9) were performed and compared with negative and positive controls. The ROS in composites were compared with NC. Statistical significance in differences was assessed using a t-test and ANOVA (α = 0.05).
RESULTS
Morphological variations in different-sized fillers were observed in the composites. The DC values were not significantly different among the composites. The amounts of 2-hydroxyethyl methacrylate (HEMA) were higher in TN than DN (p = 0.0022) and triethylene glycol dimethacrylate (TEGDMA) in CX was higher than in others (p < 0.0001). The lowest cell viability was shown in CX (p < 0.0001) and the highest ROS formation was detected in TN (p < 0.0001).
CONCLUSIONS
Three nanohybrid dental composites exhibited various compositions of filler sizes and resin components, resulting in different levels of cytotoxicity and ROS production. Chemical compositions of dental composites can be considered with their biological impact on safety issues in the intraoral use of dental restorative composites. CX with the highest TEGDMA showed the highest cytotoxicity induced by ROS accumulation. DN with lower TEGDMA and HEMA presented the highest cell viability.
背景
牙科修复复合材料的安全性问题对材料选择至关重要，但牙科医生可获得的信息有限。本研究旨在通过评估填料颗粒分析、单体转化率 (DC)、浸出物的组成以及成纤维细胞的细胞毒性和活性氧 (ROS) 产生，比较三种纳米复合牙科复合材料的化学和生物学特性。
方法
使用三种纳米复合树脂（TN、Tetric N-Ceram；CX、 Ceram X Sphere Tec One；和 DN、DenFil NX）。使用扫描电子显微镜 (SEM) 分析填料颗粒的大小分布和形态（n=5）。通过微拉曼光谱法 (µ-Raman spectroscopy) 测量 DC（n=5）。对于成分分析，使用气相色谱/质谱法 (GC/MS) 评估来自光聚合复合材料的甲醇浸出物（n=3）。将聚合后的复合材料在细胞培养基中孵育 24 小时后制备浸出物。进行死活检测（n=9）和水溶性四唑盐 1 检测（n=9），并与阴性和阳性对照进行比较。将复合材料中的 ROS 与 NC 进行比较。使用 t 检验和 ANOVA（α=0.05）评估差异的统计学显著性。
结果
在不同尺寸的填料复合材料中观察到形态变化。复合材料的 DC 值之间没有显著差异。TN 中的 2-羟乙基甲基丙烯酸酯 (HEMA) 含量高于 DN（p=0.0022），而 CX 中的三乙二醇二甲基丙烯酸酯 (TEGDMA) 含量高于其他材料（p<0.0001）。CX 显示出最低的细胞活力（p<0.0001），TN 检测到最高的 ROS 形成（p<0.0001）。
结论
三种纳米复合牙科复合材料表现出不同尺寸的填料和树脂成分的组成，导致不同程度的细胞毒性和 ROS 产生。牙科复合材料的化学成分可以考虑到其对口腔内牙科修复复合材料安全性问题的生物学影响。含有最高 TEGDMA 的 CX 显示出由 ROS 积累引起的最高细胞毒性。含较低 TEGDMA 和 HEMA 的 DN 表现出最高的细胞活力。
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