采用分批补料策略从玉米芯废渣中生产高浓度乙醇。
High concentration ethanol production from corncob residues by fed-batch strategy.
机构信息
State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shangdong University, No. 27 Shanda-nan Road, Jinan Shandong 250100, China.
出版信息
Bioresour Technol. 2010 Jul;101(13):4952-8. doi: 10.1016/j.biortech.2009.11.013. Epub 2009 Dec 9.
Ethanol production from corncob residues (CCR) pretreated by different methods was studied. The structure features of these CCR were analyzed by Fourier transform-infrared spectrum (FT-IR), X-ray diffraction (XRD), and field emission scanning electron microscope (SEM). Simultaneous saccharification and fermentation (SSF) was performed by adding crude cellulase preparations from Penicillium decumbens JUA10-1 at 30 degrees C. The results suggested that different pretreatments resulted in different composition and structure of residues; these changes had a significant influence on ethanol productivity and concentration. The fed-batch method was combined with SSF to enhance ethanol concentration further and reduce enzyme dosage. Moreover, the absorption and desorption phenomena of cellobiohydrolase I (CBH I) (70 kDa) were observed to be related to lignin contents in residues. These results demonstrated that despite the application of low enzyme dosage, high concentration ethanol could be produced from pretreated corncobs by combining fed-batch method with SSF.
研究了不同方法预处理后的玉米芯残渣（CCR）生产乙醇的情况。通过傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和场发射扫描电子显微镜（SEM）对这些 CCR 的结构特征进行了分析。通过添加来自青霉属 JUA10-1 的粗纤维素酶制剂，在 30°C 下进行同步糖化发酵（SSF）。结果表明，不同的预处理导致残渣的组成和结构不同；这些变化对乙醇产率和浓度有显著影响。采用补料分批法与 SSF 相结合，进一步提高了乙醇浓度，降低了酶用量。此外，还观察到纤维二糖水解酶 I（CBH I）（70 kDa）的吸附和解吸现象与残渣中的木质素含量有关。这些结果表明，尽管使用了低酶用量，但通过补料分批法与 SSF 相结合，仍可从预处理过的玉米芯中生产出高浓度的乙醇。
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