Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA.
Bioresour Technol. 2011 Dec;102(23):10892-7. doi: 10.1016/j.biortech.2011.09.041. Epub 2011 Sep 18.
Ethanol production by a recombinant bacterium from wheat straw (WS) at high solid loading by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) was studied. The yield of total sugars from dilute acid pretreated WS (150 g/L) after enzymatic saccharification was 86.3±1.5 g/L. The pretreated WS was bio-abated by growing a fungal strain aerobically in the liquid portion for 16 h. The recombinant Escherichia coli strain FBR5 produced 41.1±1.1 gethanol/L from non-abated WS hydrolyzate (total sugars, 86.6±0.3 g/L) in 168 h at pH7.0 and 35 °C. The bacterium produced 41.8±0.0 g ethanol/L in 120 h from the bioabated WS by SHF. It produced 41.6±0.7 g ethanol/L in 120 h from bioabated WS by fed-batch SSF. This is the first report of the production of above 4% ethanol from a lignocellulosic hydrolyzate by the recombinant bacterium.
研究了利用重组细菌在高固载量下通过分步水解发酵（SHF）和同步糖化发酵（SSF）从小麦秸秆（WS）生产乙醇。经酶解后，稀酸预处理 WS（150 g/L）中的总糖得率为 86.3±1.5 g/L。真菌菌株在液体部分好氧生长 16 小时可生物降解预处理 WS。重组大肠杆菌 FBR5 在 pH7.0 和 35°C 下，从未生物降解的 WS 水解液（总糖 86.6±0.3 g/L）中发酵 168 小时可产生 41.1±1.1 g/L 的乙醇。该细菌通过 SHF 从生物降解的 WS 中产生 41.8±0.0 g/L 的乙醇，在 120 小时内完成。通过 fed-batch SSF 从生物降解的 WS 中产生 41.6±0.7 g/L 的乙醇。这是首例利用重组细菌从木质纤维素水解物中生产 4%以上乙醇的报道。
