粪便聚合酶链反应检测儿童幽门螺杆菌及克拉霉素药敏试验。
Stool polymerase chain reaction for Helicobacter pylori detection and clarithromycin susceptibility testing in children.
机构信息
St. Anna Children's Hospital, Vienna, Austria.
出版信息
Clin Gastroenterol Hepatol. 2010 Mar;8(3):309-12. doi: 10.1016/j.cgh.2009.12.002. Epub 2009 Dec 28.
BACKGROUND & AIMS: This study was undertaken in a pediatric gastroenterology clinic to retrospectively evaluate a real-time polymerase chain reaction (PCR) for the detection and clarithromycin susceptibility testing of Helicobacter pylori using stool specimens.
METHODS
All consecutive children who underwent a gastroscopy between March 2006 and February 2009 and also having been examined by stool PCR were enrolled. Rapid urease test, histology, and culture were the reference methods for the detection of H pylori and E-test for susceptibility testing, respectively.
RESULTS
A total of 143 children (mean age, 10.8 y; range, 2.8-17.9; males:females, 1:1.5) were evaluable. Sensitivity, specificity, and test accuracy for the detection of H pylori were 83.8%, 98.4%, and 90.2%, respectively. Sensitivity, specificity, and accuracy for the detection of clarithromycin resistance were 89.2%, 100%, and 94.0%, respectively.
CONCLUSIONS
Stool PCR was a reliable and useful noninvasive tool for detection and clarithromycin susceptibility testing of H pylori in a pediatric population with a high prevalence of clarithromycin-resistant strains.
背景与目的
本研究在儿科胃肠病学诊所进行，旨在回顾性评估使用粪便标本检测和克拉霉素药敏试验的粪便实时聚合酶链反应（PCR）检测幽门螺杆菌（H. pylori）的效果。
方法
所有连续于 2006 年 3 月至 2009 年 2 月间接受胃镜检查且同时进行粪便 PCR 检查的儿童均被纳入研究。快速尿素酶试验、组织学和培养分别是 H. pylori 检测的参考方法，E 试验是药敏试验的参考方法。
结果
共有 143 名儿童（平均年龄 10.8 岁；范围 2.8-17.9；男：女，1:1.5）可进行评估。检测 H. pylori 的敏感性、特异性和试验准确性分别为 83.8%、98.4%和 90.2%。检测克拉霉素耐药性的敏感性、特异性和准确性分别为 89.2%、100%和 94.0%。
结论
粪便 PCR 是一种可靠且有用的非侵入性工具，可用于检测儿科人群中高比例克拉霉素耐药菌株的 H. pylori 及其克拉霉素药敏试验。
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