实时 PCR 粪便检测在巴西儿童幽门螺杆菌检测和克拉霉素药敏试验中的应用。
Application of real-time PCR stool assay for Helicobacter pylori detection and clarithromycin susceptibility testing in Brazilian children.
机构信息
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil.
出版信息
Helicobacter. 2011 Aug;16(4):311-5. doi: 10.1111/j.1523-5378.2011.00845.x.
BACKGROUND
Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens.
OBJECTIVE
The aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children.
METHODS
DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing.
RESULTS
The sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pylori infection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance.
CONCLUSION
This assay proved to be appropriate for H. pylori clarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.
背景
幽门螺杆菌 ClariRes 检测法是一种新型的商业化实时 PCR 检测法，可用于胃活检或粪便标本中幽门螺杆菌的检测和克拉霉素药敏试验。
目的
本研究旨在验证新型双探针实时检测法在 217 例消化不良儿童粪便标本中的应用。
方法
从胃活检和粪便标本中提取 DNA，并提交双探针实时检测法进行幽门螺杆菌检测和克拉霉素药敏试验。
结果
该检测法对幽门螺杆菌感染的检测灵敏度、特异性和检测准确性分别为 69%、100%和 93.9%，对克拉霉素耐药性的检测灵敏度、特异性和检测准确性分别为 83.3%、100%和 95.6%。
结论
该检测法适用于幽门螺杆菌克拉霉素药敏试验，特别是在疑似存在高克拉霉素耐药菌株的儿童人群中。
Zotero 插件