Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Talanta. 2010 Jan 15;80(3):1228-33. doi: 10.1016/j.talanta.2009.09.014.
In this paper, the interaction of DNA molecules with aqueous CdTe quantum dots (CdTe QDs), CdTe/SiO(2) composite nanoparticles (CdTe/SiO(2) NPs), and Mn-doped ZnSe quantum dots (Mn:ZnSe d-dots) was studied with ethidium bromide as a probe. The purpose of this work was to study the damage of DNA molecules induced by these three kinds of water-soluble nanoparticles. It was found that ionic strength, pH value and UV irradiation influenced the PL emission properties of CdTe QDs, CdTe/SiO(2) NPs and Mn:ZnSe d-dots, and also influenced the interaction of DNA molecules with them. Among the three kinds of nanoparticles, DNA molecules were most easily damaged by CdTe QDs whether in the dark or under UV irradiation. The CdTe/SiO(2) NPs led to much less DNA damage when compared with CdTe QDs, as a silica overcoating layer could isolate the QDs from the external environment. Mn:ZnSe d-dots as a new class of non-cadmium doped QDs demonstrated almost no damage for DNA molecules, which have great potentials as fluorescent labels in the applications of biomedical assays, imaging of cells and tissues, even in vivo investigations.
本文以溴化乙锭为探针研究了 DNA 分子与水相 CdTe 量子点（CdTe QDs）、CdTe/SiO2 复合纳米粒子（CdTe/SiO2 NPs）和 Mn 掺杂 ZnSe 量子点（Mn:ZnSe d-dots）的相互作用。本工作旨在研究这三种水溶性纳米粒子诱导的 DNA 分子损伤。结果表明，离子强度、pH 值和紫外辐照影响了 CdTe QDs、CdTe/SiO2 NPs 和 Mn:ZnSe d-dots 的光致发光发射特性，也影响了 DNA 分子与它们的相互作用。在这三种纳米粒子中，无论在黑暗中还是在紫外辐照下，DNA 分子最容易被 CdTe QDs 损伤。与 CdTe QDs 相比，CdTe/SiO2 NPs 导致的 DNA 损伤要小得多，因为覆盖层可以将 QDs 与外部环境隔离。Mn:ZnSe d-dots 作为一类新型的无镉掺杂 QDs，对 DNA 分子几乎没有损伤，在生物医学分析、细胞和组织成像，甚至活体研究等应用中作为荧光标记具有很大的潜力。
