Department of Chemistry, Liaoning University, Shenyang 110036, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Nov;77(4):895-901. doi: 10.1016/j.saa.2010.08.065. Epub 2010 Sep 17.
The previous work proved that the bovine serum albumin (BSA) could be damaged under the combined action of ultrasonic irradiation and ZnO. In this work, the assisted sonocatalytic damage of BSA using metronidazole (MTZ) as a sensitizer was further investigated by means of UV-vis and fluorescence spectra. The results indicated that the adding of MTZ could obviously promote the sonocatalytic damage of BSA under ultrasonic irradiation in the presence of nano-sized ZnO powder. Furthermore, it was found that the damage degree of BSA was aggravated by some influencing factors except ionic kind and strength. In addition, the damage site of BSA was also studied with synchronous fluorescence technology. It was found that the damage site was mainly at tryptophan (Trp) residue.
先前的工作证明，在超声辐射和 ZnO 的共同作用下，牛血清白蛋白（BSA）可能会受到损伤。在这项工作中，通过紫外可见光谱和荧光光谱进一步研究了甲硝唑（MTZ）作为敏化剂辅助超声催化破坏 BSA 的情况。结果表明，在纳米 ZnO 粉末存在的情况下，添加 MTZ 可以明显促进超声辐射下 BSA 的声催化损伤。此外，除了离子种类和强度之外，还发现一些影响因素会加剧 BSA 的损伤程度。此外，还通过同步荧光技术研究了 BSA 的损伤部位。结果发现，损伤部位主要在色氨酸（Trp）残基上。
