Dipartimento di Chimica, Università degli Studi di Firenze, Via della Lastruccia 3, 50019, Sesto Fiorentino, Italy.
Anal Bioanal Chem. 2008 May;391(2):455-71. doi: 10.1007/s00216-008-1876-4. Epub 2008 Feb 17.
The detection and identification of foodborne pathogens continue to rely on conventional culturing techniques. These are very elaborate, time-consuming, and have to be completed in a microbiology laboratory and are therefore not suitable for on-site monitoring. The need for a more rapid, reliable, specific, and sensitive method of detecting a target analyte, at low cost, is the focus of a great deal of research. Biosensor technology has the potential to speed up the detection, increase specificity and sensitivity, enable high-throughput analysis, and to be used for monitoring of critical control points in food production. This article reviews food pathogen detection methods based on electrochemical biosensors, specifically amperometric, potentiometric, and impedimetric biosensors. The underlying principles and application of these biosensors are discussed with special emphasis on new biorecognition elements, nanomaterials, and lab on a chip technology.
食源性致病菌的检测和鉴定仍然依赖于传统的培养技术。这些技术非常复杂、耗时，并且必须在微生物学实验室中完成，因此不适合现场监测。因此，人们需要一种更快速、可靠、特异和敏感的方法来低成本检测目标分析物，这是大量研究的重点。生物传感器技术具有加快检测速度、提高特异性和灵敏度、实现高通量分析以及用于监测食品生产中关键控制点的潜力。本文综述了基于电化学生物传感器的食源性致病菌检测方法，特别是电流型、电位型和阻抗型生物传感器。讨论了这些生物传感器的基本原理和应用，特别强调了新的生物识别元件、纳米材料和芯片实验室技术。
