2014-2017 年突尼斯莫纳斯提尔市脑脊液和污水样本中检测到的肠道病毒的分子特征。
Molecular characterization of enterovirus detected in cerebrospinal fluid and wastewater samples in Monastir, Tunisia, 2014-2017.
机构信息
Faculty of Pharmacy, Laboratory of Infectious Diseases and Biological Agents, University of Monastir, LR99-ES27, 5000, Monastir, Tunisia.
Laboratory of Microbiology, Fattouma Bourguiba University Hospital, Monastir, Tunisia.
出版信息
Virol J. 2022 Mar 18;19(1):45. doi: 10.1186/s12985-022-01770-w.
BACKGROUND
Enteroviruses (EVs) are considered the main causative agents responsible for aseptic meningitis worldwide. This study was conducted in the Monastir region of Tunisia in order to know the prevalence of EV infections in children with meningitis symptoms. Detected EV types were compared to those identified in wastewater samples.
METHODS
Two hundred CSF samples collected from hospitalized patients suspected of having aseptic meningitis for an EV infection between May 2014 and May 2017 and 80 wastewater samples collected in the same time-period were analyzed. EV detection and genotyping were performed using PCR methods followed by sequencing. Phylogenetic analyses in the 3'-VP1 region were also carried-out.
RESULTS
EVs were detected in 12% (24/200) CSF and in 35% (28/80) wastewater samples. EV genotyping was reached in 50% (12/24) CSF-positive samples and in 64% (18/28) sewage. Most frequent types detected in CSF were CVB3, E-30 and E-9 (25% each). In wastewater samples, the same EVs were identified, but also other types non-detected in CSF samples, such as E-17,CVA9 and CVB1 from EV species B, and EV-A71 and CVA8 from EV-A, suggesting their likely lower pathogenicity. Phylogenetic analysis showed that within the same type, different strains circulate in Tunisia. For some of the EV types such as E-9, E-11 or CVB3, the same strains were detected in CSF and wastewater samples.
CONCLUSIONS
Epidemiological studies are important for the surveillance of the EV infections and to better understand the emergence of certain types and variants.
背景
肠道病毒（EV）被认为是全球无菌性脑膜炎的主要致病因子。本研究在突尼斯的莫纳斯提尔地区进行，旨在了解有脑膜炎症状的儿童中 EV 感染的流行情况。所检测到的 EV 型与在废水样本中鉴定出的 EV 型进行了比较。
方法
对 2014 年 5 月至 2017 年 5 月期间因 EV 感染住院的疑似无菌性脑膜炎患者的 200 份 CSF 样本和同期收集的 80 份废水样本进行了分析。使用 PCR 方法进行 EV 检测和基因分型，然后进行测序。还对 3'-VP1 区进行了系统进化分析。
结果
在 12%（24/200）的 CSF 和 35%（28/80）的废水中检测到 EV。在 50%（12/24）的 CSF 阳性样本和 64%（18/28）的污水样本中达到了 EV 基因分型。在 CSF 中检测到的最常见类型是 CVB3、E-30 和 E-9（各占 25%）。在废水样本中，也发现了相同的 EV，但也发现了 CSF 样本中未检测到的其他类型，如 EV 种 B 中的 E-17、CVA9 和 CVB1，以及 EV-A 中的 EV-A71 和 CVA8，表明它们的致病性可能较低。系统进化分析表明，在同一类型内，不同的菌株在突尼斯循环。对于某些 EV 型，如 E-9、E-11 或 CVB3，在 CSF 和废水样本中检测到相同的菌株。
结论
流行病学研究对于 EV 感染的监测以及更好地了解某些类型和变体的出现非常重要。
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