两步方案用于快速鉴定和区分嗜肺军团菌和非嗜肺军团菌。
Two-step scheme for rapid identification and differentiation of Legionella pneumophila and non-Legionella pneumophila species.
机构信息
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, Guangdong, China.
出版信息
J Clin Microbiol. 2010 Feb;48(2):433-9. doi: 10.1128/JCM.01778-09. Epub 2009 Dec 9.
A rapid two-step scheme based on PCR amplification and enzymatic digestion analysis of a 226-bp fragment of the 16S rRNA gene was developed to identify the Legionella genus by PCR amplification and to differentiate the Legionella pneumophila and non-Legionella pneumophila species by enzymatic digestion analysis. Among 42 ATCC strains (16 strains of L. pneumophila and 26 strains of non-L. pneumophila) and 200 Legionella isolates from environmental water samples, including pools, rivers, lakes, and cooling towers in Guangdong province, 99.59% of L. pneumophila and non-L. pneumophila strains were correctly identified and differentiated by this scheme. The procedure of this two-step identification and differentiation scheme is simple and takes only about 4 h. These results suggest that this two-step scheme provides a simple and convenient method for the rapid identification and differentiation of L. pneumophila and non-L. pneumophila species.
建立了一种基于聚合酶链反应（PCR）扩增和 16S rRNA 基因 226bp 片段酶切分析的快速两步方案，用于通过 PCR 扩增鉴定军团菌属，并通过酶切分析区分肺炎军团菌和非肺炎军团菌。在 42 株 ATCC 菌株（16 株肺炎军团菌和 26 株非肺炎军团菌）和 200 株来自广东省环境水样的军团菌分离株中，该方案可正确识别和区分 99.59%的肺炎军团菌和非肺炎军团菌。该两步鉴定和区分方案的步骤简单，仅需约 4 小时。这些结果表明，该两步方案为快速鉴定和区分肺炎军团菌和非肺炎军团菌提供了一种简单方便的方法。
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