通过聚合酶链反应检测大不里士医院中的军团菌污染情况。
Detection of legionella contamination in tabriz hospitals by PCR assay.
作者信息
Ghotaslou Reza, Yeganeh Sefidan Fatemeh, Akhi Mohammad Taghi, Soroush Mohammad Hussein, Hejazi Mohammad Saeid
机构信息
Tropical and Infectious Diseases Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Adv Pharm Bull. 2013;3(1):131-4. doi: 10.5681/apb.2013.022. Epub 2013 Feb 7.
Abstract
PURPOSE
The present study was designed to evaluate the occurrence of Legionella contamination in the tap water of Tabriz hospitals, Azerbaijan, Iran.
METHODS
One hundred and forty water samples from diverse water supply systems of 17 hospitals were collected and analyzed for the presence of Legionella spp. by PCR assay.
RESULTS
In this study, 10 of 140 (7.1%) samples were positive for Legionella which L. pneumophila was detected in 4 (2.85%) water samples.
CONCLUSION
In conclusion, hospital potable systems are the primary reservoirs for Legionnaires' disease. This study concludes that Legionella spp. are present in aquatic hospitals environment of Tabriz. Due to the serious risk of infections, it is better to make efforts to eliminate Legionella spp. in water supplies.
摘要
目的
本研究旨在评估伊朗阿塞拜疆大不里士市医院自来水中军团菌污染的发生情况。
方法
从17家医院的不同供水系统中采集了140份水样，并通过聚合酶链反应（PCR）检测法分析其中军团菌属的存在情况。
结果
在本研究中，140份样本中有10份（7.1%）军团菌检测呈阳性，其中4份（2.85%）水样检测到嗜肺军团菌。
结论
总之，医院饮用水系统是军团病的主要储存源。本研究得出结论，大不里士市医院的水生环境中存在军团菌属。由于感染风险严重，最好努力消除供水系统中的军团菌属。
相似文献
1
Detection of legionella contamination in tabriz hospitals by PCR assay.通过聚合酶链反应检测大不里士医院中的军团菌污染情况。
Adv Pharm Bull. 2013;3(1):131-4. doi: 10.5681/apb.2013.022. Epub 2013 Feb 7.
2
Hospital characteristics associated with colonization of water systems by Legionella and risk of nosocomial legionnaires' disease: a cohort study of 15 hospitals.与军团菌在水系统中定植及医院获得性军团病风险相关的医院特征：15家医院的队列研究
Infect Control Hosp Epidemiol. 1999 Dec;20(12):798-805. doi: 10.1086/501587.
3
Prevalence of mip virulence gene and PCR-base sequence typing of Legionella pneumophila from cooling water systems of two cities in Iran.伊朗两个城市冷却水系统中嗜肺军团菌的mip毒力基因流行率及基于PCR的序列分型
Virulence. 2016 Jul 3;7(5):602-9. doi: 10.1080/21505594.2016.1170944. Epub 2016 Mar 30.
4
The high prevalence of Legionella pneumophila contamination in hospital potable water systems in Taiwan: implications for hospital infection control in Asia.台湾医院饮用水系统中嗜肺军团菌污染的高流行率：对亚洲医院感染控制的启示。
Int J Infect Dis. 2008 Jul;12(4):416-20. doi: 10.1016/j.ijid.2007.11.008. Epub 2008 Feb 20.
5
Factors Influencing Contamination of Domestic Household Showers.影响家庭淋浴器污染的因素。
Pathogens. 2019 Feb 26;8(1):27. doi: 10.3390/pathogens8010027.
6
Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR.采用定量实时荧光定量PCR技术检测医院水样中的军团菌。
Appl Environ Microbiol. 2001 Sep;67(9):3985-93. doi: 10.1128/AEM.67.9.3985-3993.2001.
7
Legionella contamination of hospital water supplies: monitoring of private healthcare facilities in Bologna, Italy.医院供水系统中的军团菌污染：意大利博洛尼亚私立医疗机构的监测
J Hosp Infect. 2002 Mar;50(3):220-3. doi: 10.1053/jhin.2002.1180.
8
Isolation and identification of spp. from different aquatic sources in south-west of Iran by molecular &culture methods.通过分子和培养方法从伊朗西南部不同水生来源分离和鉴定 spp.
Mol Biol Res Commun. 2016 Dec;5(4):215-223.
9
10
Legionella spp., amoebae and not-fermenting Gram negative bacteria in an Italian university hospital water system.意大利一家大学医院水系统中的军团菌属、变形虫和非发酵革兰氏阴性菌。
Ann Agric Environ Med. 2014;21(3):489-93. doi: 10.5604/12321966.1120623.
引用本文的文献
1
Risk assessment and quantitative measurement along with monitoring of in hospital water sources.医院水源的风险评估、定量测量及监测。
New Microbes New Infect. 2021 Dec 24;45:100948. doi: 10.1016/j.nmni.2021.100948. eCollection 2022 Jan.
2
Isolation and Identification of spp. in environmental water sources based on macrophage infectivity potentiator () gene sequencing in southwest Iran.基于巨噬细胞感染增强因子（）基因测序对伊朗西南部环境水源中 spp. 的分离与鉴定
AIMS Microbiol. 2019 Aug 16;5(3):223-231. doi: 10.3934/microbiol.2019.3.223. eCollection 2019.
3
Prevalence of Species in Water Resources of Iran: A Systematic Review and Meta-Analysis.伊朗水资源中物种的流行情况：系统评价与荟萃分析。
Iran J Med Sci. 2018 Nov;43(6):571-580.
4
Detection of Legionella Pneumophila in Urine and Serum Specimens of Neutropenic Febrile Patients with Haematological Malignancies.血液系统恶性肿瘤中性粒细胞减少发热患者尿液和血清标本中嗜肺军团菌的检测
Int J Hematol Oncol Stem Cell Res. 2017 Jan 1;11(1):49-53.
5
Isolation and identification of spp. from different aquatic sources in south-west of Iran by molecular &culture methods.通过分子和培养方法从伊朗西南部不同水生来源分离和鉴定 spp.
Mol Biol Res Commun. 2016 Dec;5(4):215-223.
6
Predictive parameters of Legionella pneumophila occurrence in hospital water: HPCs and plumbing system installation age.军团菌在医院水中发生的预测参数：HPCs 和管道系统安装年龄。
Environ Monit Assess. 2016 Sep;188(9):536. doi: 10.1007/s10661-016-5554-2. Epub 2016 Aug 30.
7
Etiological Diagnosis of Community-Acquired Pneumonia in Adult Patients: A Prospective Hospital-Based Study in Mashhad, Iran.成年患者社区获得性肺炎的病因诊断：一项在伊朗马什哈德开展的基于医院的前瞻性研究
Jundishapur J Microbiol. 2015 Aug 29;8(8):e22780. doi: 10.5812/jjm.22780. eCollection 2015 Aug.
本文引用的文献
1
High diversity and abundance of Legionella spp. in a pristine river and impact of seasonal and anthropogenic effects.高多样性和丰度的军团菌属在原始河流中的存在，以及季节性和人为因素的影响。
Appl Environ Microbiol. 2010 Dec;76(24):8201-10. doi: 10.1128/AEM.00188-10. Epub 2010 Oct 22.
2
Effective environmental sampling strategies for monitoring Legionella spp contamination in hot water systems.有效环境采样策略监测热水系统中军团菌属污染。
Am J Infect Control. 2010 Jun;38(5):344-9. doi: 10.1016/j.ajic.2009.09.016. Epub 2010 Jan 18.
3
Two-step scheme for rapid identification and differentiation of Legionella pneumophila and non-Legionella pneumophila species.两步方案用于快速鉴定和区分嗜肺军团菌和非嗜肺军团菌。
J Clin Microbiol. 2010 Feb;48(2):433-9. doi: 10.1128/JCM.01778-09. Epub 2009 Dec 9.
4
Microbial characterization of water and biofilms in drinking water distribution systems at sport facilities.
Cent Eur J Public Health. 2009 Jun;17(2):99-102. doi: 10.21101/cejph.a3511.
5
A PCR-based method for monitoring Legionella pneumophila in water samples detects viable but noncultivable legionellae that can recover their cultivability.一种基于聚合酶链式反应（PCR）的方法用于监测水样中的嗜肺军团菌，该方法可检测出具有活力但不可培养的军团菌，这些军团菌能够恢复其可培养性。
Appl Environ Microbiol. 2008 Aug;74(15):4817-24. doi: 10.1128/AEM.02899-07. Epub 2008 May 30.
6
The high prevalence of Legionella pneumophila contamination in hospital potable water systems in Taiwan: implications for hospital infection control in Asia.台湾医院饮用水系统中嗜肺军团菌污染的高流行率：对亚洲医院感染控制的启示。
Int J Infect Dis. 2008 Jul;12(4):416-20. doi: 10.1016/j.ijid.2007.11.008. Epub 2008 Feb 20.
7
Role of environmental surveillance in determining the risk of hospital-acquired legionellosis: a national surveillance study with clinical correlations.环境监测在确定医院获得性军团菌病风险中的作用：一项具有临床相关性的全国性监测研究
Infect Control Hosp Epidemiol. 2007 Jul;28(7):818-24. doi: 10.1086/518754. Epub 2007 Jun 5.
8
Quantitative real-time Legionella PCR for environmental water samples: data interpretation.环境水样中军团菌的定量实时聚合酶链反应：数据解读
Appl Environ Microbiol. 2006 Apr;72(4):2801-8. doi: 10.1128/AEM.72.4.2801-2808.2006.
9
Environmental cultures and hospital-acquired Legionnaires' disease: a 5-year prospective study in 20 hospitals in Catalonia, Spain.环境培养与医院获得性军团病：西班牙加泰罗尼亚20家医院的5年前瞻性研究。
Infect Control Hosp Epidemiol. 2004 Dec;25(12):1072-6. doi: 10.1086/502346.
10
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species.一种用于检测嗜肺军团菌及其他军团菌属的内控单管多重实时荧光定量PCR检测方法的研发与临床评估
J Clin Microbiol. 2003 Sep;41(9):4016-21. doi: 10.1128/JCM.41.9.4016-4021.2003.
Zotero 插件