University of Alabama at Birmingham, School of Medicine, Department of Pathology, Room P230, 619 19th St., South, Birmingham, AL 35249-7331, USA.
J Clin Microbiol. 2010 Feb;48(2):599-602. doi: 10.1128/JCM.01828-09. Epub 2009 Dec 9.
Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.
脉冲场凝胶电泳(PFGE)被认为是分子流行病学研究的“金标准”。重复回文外扩增 PCR(rep-PCR)耗时更少,更适合分析人群中大量的细菌菌株。PFGE 和 rep-PCR 为调查变形链球菌多样性和传播提供了可比的基因分型结果。