重复外回文 PCR 研究人群中变异链球菌的多样性和传播。
Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations.
机构信息
University of Alabama at Birmingham, School of Medicine, Department of Pathology, Room P230, 619 19th St., South, Birmingham, AL 35249-7331, USA.
出版信息
J Clin Microbiol. 2010 Feb;48(2):599-602. doi: 10.1128/JCM.01828-09. Epub 2009 Dec 9.
Abstract
Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.
摘要
脉冲场凝胶电泳（PFGE）被认为是分子流行病学研究的“金标准”。重复回文外扩增 PCR（rep-PCR）耗时更少，更适合分析人群中大量的细菌菌株。PFGE 和 rep-PCR 为调查变形链球菌多样性和传播提供了可比的基因分型结果。
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