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本文引用的文献

1
Maternal transmission of mutans Streptococci in severe-early childhood caries.变形链球菌在重度幼儿早期龋齿中的母婴传播
Pediatr Dent. 2009 May-Jun;31(3):193-201.
2
Association of mutans streptococci between caregivers and their children.照顾者及其子女之间变形链球菌的关联。
Pediatr Dent. 2008 Sep-Oct;30(5):375-87.
3
The etiology of periodontal disease revisited by population genetic analysis.通过群体遗传分析重新审视牙周病的病因。
Periodontol 2000. 2006;42:158-79. doi: 10.1111/j.1600-0757.2006.00159.x.
4
DNA fingerprinting of isolates of Streptococcus mutans by pulsed-field gel electrophoresis.采用脉冲场凝胶电泳对变形链球菌分离株进行DNA指纹分析。
Microbiol Res. 2007;162(3):244-9. doi: 10.1016/j.micres.2006.06.014. Epub 2006 Jul 25.
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Genotypic analysis of strains of mutans streptococci by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳对变形链球菌菌株进行基因分型分析。
Microbiol Res. 2004;159(3):181-6. doi: 10.1016/j.micres.2004.01.016.
6
Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus.基于5'核酸酶的实时荧光定量PCR检测方法的建立，用于定量检测致龋性口腔病原菌变形链球菌和远缘链球菌。
J Clin Microbiol. 2003 Sep;41(9):4438-41. doi: 10.1128/JCM.41.9.4438-4441.2003.
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Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O157:H7.评估脉冲场凝胶电泳作为确定大肠杆菌O157:H7菌株间遗传相关性程度的工具。
J Clin Microbiol. 2003 May;41(5):1843-9. doi: 10.1128/JCM.41.5.1843-1849.2003.
8
Tracing genotypes of mutans streptococci on tooth sites by random amplified polymorphic DNA (RAPD) analysis.通过随机扩增多态性DNA（RAPD）分析追踪变形链球菌在牙齿部位的基因型。
Oral Microbiol Immunol. 2003 Feb;18(1):24-9. doi: 10.1034/j.1399-302x.2002.180104.x.
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How xylitol-containing products affect cariogenic bacteria.含木糖醇的产品如何影响致龋菌。
J Am Dent Assoc. 2002 Apr;133(4):435-41; quiz 492-3. doi: 10.14219/jada.archive.2002.0201.
10
Influences of orthodontic appliances on oral populations of mutans streptococci.正畸矫治器对变形链球菌口腔菌群的影响。
Oral Microbiol Immunol. 2002 Apr;17(2):65-71. doi: 10.1046/j.0902-0055.2001.00083.x.

重复外回文 PCR 研究人群中变异链球菌的多样性和传播。

Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations.

机构信息

University of Alabama at Birmingham, School of Medicine, Department of Pathology, Room P230, 619 19th St., South, Birmingham, AL 35249-7331, USA.

出版信息

J Clin Microbiol. 2010 Feb;48(2):599-602. doi: 10.1128/JCM.01828-09. Epub 2009 Dec 9.

DOI:10.1128/JCM.01828-09
PMID:20007400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2815590/
Abstract

Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.

摘要

脉冲场凝胶电泳（PFGE）被认为是分子流行病学研究的“金标准”。重复回文外扩增 PCR（rep-PCR）耗时更少，更适合分析人群中大量的细菌菌株。PFGE 和 rep-PCR 为调查变形链球菌多样性和传播提供了可比的基因分型结果。