Department of Medical Microbiology and Hygiene, Gärtner & Colleagues Laboratories, Elisabethenstrasse 11, 88212 Ravensburg, Germany.
Department of Medical Microbiology, Labor Clotten, Bismarckallee 10, 79098 Freiburg/Breisgau, Germany.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2544-2547. doi: 10.1099/ijs.0.019927-0. Epub 2009 Dec 11.
A non-lipophilic, coryneform bacterium isolated from a patient's wound caused by a dog bite was characterized by phenotypic, chemotaxonomic and molecular genetic methods. Chemotaxonomic features suggested assignment of the unknown bacterium to the genus Corynebacterium. The isolate exhibited the following unusual features, which made it possible to phenotypically differentiate it from all other medically relevant corynebacteria: the Gram stain showed some very filamentous rods (>15 μm in length); some cells exhibited branching; colonies were domed and adherent to agar; the micro-organism was positive for pyrazinamidase, β-glucosidase, α-glucosidase and trypsin but negative for β-galactosidase. 16S rRNA gene sequencing and partial rpoB gene sequencing showed that the closest phylogenetic relative, Corynebacterium freiburgense, exhibited more than 1.9 % and 17.9 % divergence with the unknown bacterium, respectively. Based on both phenotypic and molecular genetic data, it is proposed that the isolate should be classified as a novel species, Corynebacterium canis sp. nov., with the type strain 1170(T) (=CCUG 58627(T) =DSM 45402(T)).
从犬咬伤患者伤口中分离出一株非脂溶性棒状杆菌，采用表型、化学分类和分子遗传学方法对其进行了鉴定。化学分类特征表明，该未知细菌被归为棒状杆菌属。该分离株具有以下不寻常的特征，使其能够在表型上与所有其他与医学相关的棒状杆菌区分开来：革兰氏染色显示出一些非常长的丝状棒状菌（>15 μm）；一些细胞表现出分枝；菌落呈圆顶状，紧贴琼脂；该微生物对吡嗪酰胺酶、β-葡萄糖苷酶、α-葡萄糖苷酶和胰蛋白酶呈阳性，但对β-半乳糖苷酶呈阴性。16S rRNA 基因测序和部分 rpoB 基因测序显示，最接近的系统发育相关细菌，即弗雷堡棒状杆菌，与未知细菌的 16S rRNA 基因序列差异分别超过 1.9%和 17.9%。基于表型和分子遗传学数据，建议将该分离株分类为一个新的物种，即犬棒状杆菌（Corynebacterium canis sp. nov.），其模式株为 1170T（=CCUG 58627T=DSM 45402T）。
