转录组分析异养、自养和混合营养生长支持极端嗜热嗜酸古菌 Metallosphaera sedula 的生理多样性。
Physiological versatility of the extremely thermoacidophilic archaeon Metallosphaera sedula supported by transcriptomic analysis of heterotrophic, autotrophic, and mixotrophic growth.
机构信息
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
出版信息
Appl Environ Microbiol. 2010 Feb;76(3):931-5. doi: 10.1128/AEM.01336-09. Epub 2009 Dec 11.
Abstract
Comparative transcriptomic analysis of autotrophic, heterotrophic, and mixotrophic growth of the archaeon Metallosphaera sedula (70 degrees C, pH 2.0) revealed candidates for yet-to-be-confirmed components of the 3-hydroxypropionate/4-hydroxybutyrate pathway and implicated a membrane-bound hydrogenase (Msed_0944-Msed_0946) for growth on H(2). Routes for generation of ATP and reducing equivalents were also identified.
摘要
对自养、异养和混养生长的古菌 Metallosphaera sedula（70°C，pH 2.0）的比较转录组分析揭示了 3-羟基丙酸/4-羟基丁酸途径中尚未得到证实的成分的候选物，并暗示了一种膜结合氢化酶（Msed_0944-Msed_0946）可用于 H2 生长。还确定了生成 ATP 和还原当量的途径。
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