Ding Shou-Nian, Shan Dan, Xue Huai-Guo, Zhu Dao-Bin, Cosnier Serge
School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, P. R. China.
Anal Sci. 2009 Dec;25(12):1421-5. doi: 10.2116/analsci.25.1421.
A new type of amperometric glucose biosensor based on alginate (Alg)/layered double hydroxides (LDHs) organic-inorganic composite film is described. This hybrid material combines the advantages of an organic biopolymer, Alg and inorganic LDHs. Glucose oxidase (GOD) immobilized in the material maintained its activity. The composite films were characterized by UV-Vis. The results indicated that GOD retained the essential feature of its native structure in the composite film. The Alg/LDHs/GOD-modified platinum electrode exhibited a fast response to glucose (achieve 95% of the maximum in 10 s), which provided a linear response to glucose over a concentration range of 1.6 x 10(-5)-2 x 10(-3) M with a detection limit of 4 x 10(-5) M based on S/N = 3. Furthermore, the biosensor exhibited excellent long-term stability, and satisfactory reproducibility. It retained 87% of its original activity after being used for 28 days.
描述了一种基于藻酸盐（Alg）/层状双氢氧化物（LDHs）有机-无机复合膜的新型安培型葡萄糖生物传感器。这种杂化材料结合了有机生物聚合物Alg和无机LDHs的优点。固定在该材料中的葡萄糖氧化酶（GOD）保持了其活性。复合膜通过紫外-可见光谱进行表征。结果表明，GOD在复合膜中保留了其天然结构的基本特征。Alg/LDHs/GOD修饰的铂电极对葡萄糖表现出快速响应（10 s内达到最大值的95%），在1.6×10⁻⁵ - 2×10⁻³ M的葡萄糖浓度范围内提供线性响应，基于信噪比S/N = 3时的检测限为4×10⁻⁵ M。此外，该生物传感器表现出优异的长期稳定性和令人满意的重现性。使用28天后，它仍保留其原始活性的87%。
