Shi Qiaofang, Han En, Shan Dan, Yao Wenjuan, Xue Huaiguo
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China.
Bioprocess Biosyst Eng. 2008 Oct;31(6):519-26. doi: 10.1007/s00449-007-0190-4. Epub 2008 Jan 4.
This paper aimed at showing the interest of the composite material based on layered double hydroxides (LDHs) and chitosan (CHT) as suitable host matrix likely to immobilize enzyme onto electrode surface for amperometric biosensing application. This hybrid material combined the advantages of inorganic LDHs and organic biopolymer, CHT. Glucose oxidase (GOD) immobilized in the composite material maintained its activity well as the usage of glutaraldehyde was avoided. The process parameters for the fabrication of the enzyme electrode and various experimental variables such as pH, applied potential and temperature, were explored for optimum analytical performance of the enzyme electrode. The enzyme electrode provided a linear response to glucose over a concentration range of 1 x 10(-6) to 3 x 10(-3) M with a high sensitivity of 62.6 mA M(-1) cm(-2) and a detection limit of 0.1 microM based on the signal-to-noise ratio of 3.
本文旨在展示基于层状双氢氧化物（LDHs）和壳聚糖（CHT）的复合材料作为合适的主体基质的优势，该基质可能将酶固定在电极表面用于安培生物传感应用。这种杂化材料结合了无机LDHs和有机生物聚合物CHT的优点。固定在复合材料中的葡萄糖氧化酶（GOD）保持了良好的活性，因为避免了使用戊二醛。为了使酶电极具有最佳分析性能，研究了酶电极制备的工艺参数以及各种实验变量，如pH值、施加电位和温度。基于3的信噪比，该酶电极在1×10⁻⁶至3×10⁻³ M的葡萄糖浓度范围内提供线性响应，灵敏度高达62.6 mA M⁻¹ cm⁻²，检测限为0.1 μM。
