系统误差对不可逆酶抑制进程曲线分析的影响。
The effects of systematic errors on the analysis of irreversible enzyme inhibition progress curves.
作者信息
Gray P
机构信息
Materials Research Laboratory, Defence Science and Technology Organisation, Ascot Vale, Victoria, Australia.
出版信息
Biochem J. 1991 Feb 15;274 ( Pt 1)(Pt 1):181-5. doi: 10.1042/bj2740181.
Abstract
The effects of errors in Km and Vmax. on the determination of the kinetic constants for the irreversible inhibition of acetylcholinesterase were determined. The results show that small errors in the values of Km and Vmax. give rise to large errors in the fitted parameters k2 and Kd. The problem may be minimized for multiple progress curves by selecting an appropriate number and distribution of inhibitor concentrations.
摘要
测定了Km和Vmax误差对不可逆抑制乙酰胆碱酯酶动力学常数测定的影响。结果表明，Km和Vmax值的微小误差会导致拟合参数k2和Kd出现较大误差。通过选择合适数量和分布的抑制剂浓度，对于多条进程曲线，该问题可能会最小化。
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本文引用的文献
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Kinetics for the inhibition of acetylcholinesterase from the electric eel by some organophosphates and carbamates.某些有机磷酸酯和氨基甲酸酯对电鳗乙酰胆碱酯酶的抑制动力学。
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