突触的定量神经蛋白质组学
Quantitative neuroproteomics of the synapse.
作者信息
Ramos-Ortolaza Dinah Lee, Bushlin Ittai, Abul-Husn Noura, Annangudi Suresh P, Sweedler Jonathan, Devi Lakshmi A
机构信息
Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY, USA.
出版信息
Methods Mol Biol. 2010;615:227-46. doi: 10.1007/978-1-60761-535-4_18.
Abstract
An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.
摘要
一种研究神经精神疾病或神经退行性疾病的新方法是对脑组织进行蛋白质组学分析。在这里，我们描述了用于分离和鉴定与感兴趣的样本（如突触）相关的蛋白质的方法，以及比较不同条件下样本中蛋白质水平的方法。这些技术，包括亚细胞分级分离和使用同位素标记的现代定量蛋白质组学，可用于了解神经元区室的组织以及各种条件下突触功能的调节。
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