van Boxtel Ruben, Gould Michael N, Cuppen Edwin, Smits Bart M G
Hubrect Institute, Niob Knaw, Utrecht, The Netherlands.
Methods Mol Biol. 2010;597:151-67. doi: 10.1007/978-1-60327-389-3_11.
The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.
大鼠是生物医学研究中最常用的模式生物之一，对于将生理学和病理学与基因组联系起来极为有用。然而，在大鼠生殖系中对特定基因进行基因改造的方法仍然相对较少。迄今为止，生成转基因大鼠最有效的方法是基于目标选择的N-乙基-N-亚硝基脲（ENU）诱变技术。在此，我们描述了大鼠模式生物中ENU诱变和突变体检索的详细方案。
