Division of Applied Life Sciences, BK21 Program, IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Korea. [corrected]
Biotechnol Lett. 2010 Apr;32(4):571-6. doi: 10.1007/s10529-009-0185-0. Epub 2009 Dec 16.
Plant 2-Cys peroxiredoxins (2-Cys Prx) has both peroxidase and chaperon function. We overexpressed an Arabidopsis 2-Cys Prx in transgenic tall fescue (Festuca arundinacea) plants to confer tolerance against heat and methyl viologen (MV) stress. Transgenic plants were generated by Agrobacterium-mediated genetic transformation, and integration and expression of the transgene was confirmed by Southern, northern and western blot analyses. Compared to control plants, transgenic plants had significantly less electrolyte leakage and thiobarbituric acid-reactive substances (TBARS) when exposed to heat or MV. Under heat stress (42 degrees C), transgenic plants maintained their chlorophyll fluorescence (Fv/Fm) for 24 h while control plants lost chlorophyll fluorescence very quickly. We conclude that the high levels of 2-Cys Prx proteins in transgenic plants protect leaves from oxidative damage probably due to chaperon activity.
植物 2-Cys 过氧化物酶（2-Cys Prx）具有过氧化物酶和伴侣功能。我们在转基因高羊茅（Festuca arundinacea）植物中过表达了拟南芥 2-Cys Prx，以赋予其对热和甲基紫精（MV）胁迫的耐受性。通过根癌农杆菌介导的遗传转化生成了转基因植物，并通过 Southern、northern 和 western blot 分析证实了转基因的整合和表达。与对照植物相比，暴露于热或 MV 下时，转基因植物的电解质渗漏和硫代巴比妥酸反应物质（TBARS）明显减少。在热胁迫（42°C）下，转基因植物在 24 小时内保持叶绿素荧光（Fv/Fm），而对照植物的叶绿素荧光很快丧失。我们得出结论，转基因植物中高水平的 2-Cys Prx 蛋白可能由于伴侣活性而保护叶片免受氧化损伤。
