蚯蚓（赤子爱胜蚓）中的糖胺聚糖。
Glycosaminoglycans from earthworms (Eisenia andrei).
机构信息
Natural Products Research Institute, College of Pharmacy, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151-742, Republic of Korea.
出版信息
Glycoconj J. 2010 Feb;27(2):249-57. doi: 10.1007/s10719-009-9273-6. Epub 2009 Dec 16.
The whole tissue of the earthworm (Eisenia andrei) was lyophilized and extracted to purify glycosaminoglycans. Fractions, eluting from an anion-exchange column at 1.0 M and 2.0 M NaCl, showed the presence of acidic polysaccharides on agarose gel electrophoresis. Monosaccharide compositional analysis showed that galactose and glucose were most abundant monosaccharides in both fractions. Depolymerization of the polysaccharide mixture with glycosaminoglycan-degrading enzymes confirmed the presence of chondroitin sulfate/dermatan sulfate and heparan sulfate in the 2.0 M NaCl fraction. The content of GAGs (uronic acid containing polysaccharide) in the 2.0 M NaCl fraction determined by carbazole assay was 2%. Disaccharide compositional analysis using liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) analysis after chondroitinase digestion (ABC and ACII), showed that the chondroitin sulfate/dermatan sulfate contained a 4-O-sulfo (76%), 2,4-di-O-sulfo (15%), 6-O-sulfo (6%), and unsulfated (4%) uronic acid linked N-acetylgalactosamine residues. LC-ESI-MS analysis of heparin lyase I/II/III digests demonstrated the presence of N-sulfo (69%), N-sulfo-6-O-sulfo (25%) and 2-O-sulfo-N-sulfo-6-O-sulfo (5%) uronic acid linked N-acetylglucosamine residues.
将整条蚯蚓（赤子爱胜蚓）冻干并提取以纯化糖胺聚糖。从阴离子交换柱以 1.0 M 和 2.0 M NaCl 洗脱的级分在琼脂糖凝胶电泳中显示存在酸性多糖。单糖组成分析表明，两种级分中均以半乳糖和葡萄糖为最丰富的单糖。糖胺聚糖降解酶对多糖混合物的解聚证实了 2.0 M NaCl 级分中存在硫酸软骨素/硫酸皮肤素和肝素硫酸。咔唑法测定的 2.0 M NaCl 级分中 GAGs（含糖醛酸的多糖）含量为 2%。用 ABC 和 ACII 软骨素酶消化后进行液相色谱-电喷雾电离质谱（LC-ESI-MS）分析的二糖组成分析表明，硫酸软骨素/硫酸皮肤素含有 4-O-磺酸（76%）、2,4-二-O-磺酸（15%）、6-O-磺酸（6%）和非磺酸化（4%）的连接 N-乙酰半乳糖胺残基的糖醛酸。肝素酶 I/II/III 消化的 LC-ESI-MS 分析表明存在 N-磺酸（69%）、N-磺酸-6-O-磺酸（25%）和 2-O-磺酸-N-磺酸-6-O-磺酸（5%）连接 N-乙酰葡萄糖胺残基的糖醛酸。
Zotero 插件