黑曲霉在葡萄和葡萄干上产生伏马菌素 B2 和 B4。

Production of Fumonisin B2 and B4 by Aspergillus niger on grapes and raisins.

机构信息

Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Soltofts Plads 221, DK-2800 Kgs Lyngby, Denmark.

出版信息

J Agric Food Chem. 2010 Jan 27;58(2):954-8. doi: 10.1021/jf903116q.

DOI:10.1021/jf903116q

PMID:20014861

Abstract

The recent discovery of fumonisin production in Aspergillus niger, raises concerns about the presence of these mycotoxins in grapes and raisins as well as other commodities where A. niger is a frequent contaminant. Here we investigate the potential production of fumonisins in A. niger cultured on grapes and raisins. Sixty-six A. niger, 4 A. tubingensis, and 16 A. acidus strains isolated from raisins were tested for fumonisin production on laboratory media. Neither A. tubingensis nor A. acidus strains produced fumonisins, but 77% of A. niger strains did. None of the strains produced ochratoxin A. Ten selected fumonisin producing A. niger strains were further able to produce fumonisin B(2) and fumonisin B(4) on grapes in the range 171-7841 microg fumonisin B(2)/kg and 14-1157 microg fumonisin B(4)/kg. Four selected strains were able to produce fumonisin B(2) (5-6476 microg/kg) and fumonisin B(4) (12-672 microg/kg) on raisins.

摘要

最近发现黑曲霉会产生伏马菌素，这引起了人们对黑曲霉频繁污染的葡萄和葡萄干以及其他商品中是否存在这些霉菌毒素的担忧。在这里，我们研究了在葡萄和葡萄干上培养的黑曲霉产生伏马菌素的潜力。从葡萄干中分离出的 66 株黑曲霉、4 株构巢曲霉和 16 株栖土曲霉被检测是否能在实验室培养基上产生伏马菌素。构巢曲霉和栖土曲霉都没有产生伏马菌素，但 77%的黑曲霉产生了伏马菌素。没有菌株产生赭曲霉毒素 A。10 株选定的能产生伏马菌素的黑曲霉菌株在葡萄上进一步产生伏马菌素 B（2）和伏马菌素 B（4），范围为 171-7841μg 伏马菌素 B（2）/kg 和 14-1157μg 伏马菌素 B（4）/kg。4 株选定的菌株能够在葡萄干上产生伏马菌素 B（2）（5-6476μg/kg）和伏马菌素 B（4）（12-672μg/kg）。