CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 Jan;26(1):94-100. doi: 10.1080/02652030802366090.
During 2006 and 2007, a total of 64 Thai dried coffee bean samples (Coffea arabica) from two growing sites in Chiangmai Province and 32 Thai dried coffee bean samples (Coffea canephora) from two growing sites in Chumporn Province, Thailand, were collected and assessed for fumonisin contamination by black Aspergilli. No Fusarium species known to produce fumonisin were detected, but black Aspergilli had high incidences on both Arabica and Robusta Thai coffee beans. Liquid chromatography (LC) with high-resolution mass spectrometric (HRMS) detection showed that 67% of Aspergillus niger isolates from coffee beans were capable of producing fumonisins B(2) (FB(2)) and B(4) when grown on Czapek Yeast Agar with 5% NaCl. Small amounts (1-9.7 ng g(-1)) of FB(2) were detected in seven of 12 selected coffee samples after ion-exchange purification and LC-MS/MS detection. Two samples also contained FB(4). This is the first record of freshly isolated A. niger strains producing fumonisins and the first report on the natural occurrence of FB(2) and FB(4) in coffee.
在 2006 年和 2007 年，共采集了来自泰国清迈省两个种植区的 64 份泰国产干燥咖啡豆（阿拉比卡咖啡）和来自泰国春蓬省两个种植区的 32 份泰国产干燥咖啡豆（罗布斯塔咖啡）样本，评估了由黑曲霉引起的伏马菌素污染情况。未检测到已知能产生伏马菌素的镰刀菌属物种，但黑曲霉在阿拉比卡和罗布斯塔泰国咖啡豆上的发生率很高。液相色谱（LC）与高分辨率质谱（HRMS）检测显示，在含 5%NaCl 的察氏酵母琼脂上生长时，67%的咖啡豆中分离出的黑曲霉能够产生伏马菌素 B（2）（FB（2））和 B（4）。经过离子交换纯化和 LC-MS/MS 检测，在 12 个选定的咖啡样本中，有 7 个样本中检测到少量的 FB（2）（1-9.7ng g（-1））。两个样本还含有 FB（4）。这是首次记录新鲜分离的黑曲霉菌株产生伏马菌素的情况，也是首次报告咖啡中存在 FB（2）和 FB（4）的情况。
