Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al-Ain, United Arab Emirates.
Peptides. 2010 Apr;31(4):548-54. doi: 10.1016/j.peptides.2009.12.013. Epub 2009 Dec 14.
Peptidomic analysis of norepinephrine-stimulated skin secretions of the South-East Asian frog Hylarana erythraea (formerly Rana erythraea partim) has led to the identification of multiple peptides with antimicrobial activity. Structural characterization of the peptides demonstrated that they belong to the brevinin-1 (3), brevinin-2 (2), esculentin-2 (4), and temporin (1) families. The values in parentheses indicate the number of paralogs. In addition, a peptide (GVIKSVLKGVAKTVALG ML.NH(2)) was isolated that shows some structural similarity to the brevinin-2-related peptides (B2RP) previously isolated from North American frogs of the genus Lithobates. A synthetic replicate of the species B2RP showed broad-spectrum growth inhibitory activity against reference strains of Escherichia coli (MIC=12.5 microM), Staphylococcus aureus (MIC=12.5 microM) and Candida albicans (MIC=50 microM) and was active against multidrug-resistant clinical isolates of Acetinobacter baumannii (MIC in the range 6-12.5 microM). The hemolytic activity of the peptide was relatively low (LC(50)=280 microM). Phylogenetic analysis based upon the amino acid sequences of 47 brevinin-2 peptides from 17 Asian species belonging to the family Ranidae provides support for the placement of H. erythraea in the genus Hylarana.
对东南亚青蛙（原称红蹼树蛙）去甲肾上腺素刺激的皮肤分泌物进行肽组学分析，鉴定出多种具有抗菌活性的肽。对这些肽的结构特征分析表明，它们属于breviin-1（3）、breviin-2（2）、esculetin-2（4）和temporin（1）家族。括号中的数字表示同系物的数量。此外，还分离出一种肽（GVIKSVLKGVAKTVALG ML.NH(2)），它与先前从北美的 Lithobates 属蛙中分离出的 brevinin-2 相关肽（B2RP）具有一定的结构相似性。B2RP 的合成复制品对大肠杆菌（MIC=12.5 microM）、金黄色葡萄球菌（MIC=12.5 microM）和白色念珠菌（MIC=50 microM）的参考菌株表现出广谱的生长抑制活性，对多药耐药的鲍曼不动杆菌临床分离株也具有活性（MIC 在 6-12.5 microM 范围内）。该肽的溶血活性相对较低（LC(50)=280 microM）。基于 17 种属于 Ranidae 科的亚洲物种的 47 种 brevinin-2 肽的氨基酸序列进行的系统发育分析，支持将 H. erythraea 归入 Hylarana 属。
