Brown J L, Sonoda S, Ueda H, Scott M P, Wu C
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
EMBO J. 1991 Mar;10(3):665-74. doi: 10.1002/j.1460-2075.1991.tb07995.x.
The striped expression of the Drosophila segmentation gene fushi tarazu in alternate parasegments of the early embryo is controlled by the 740 bp zebra element. Among multiple protein factors that bind to the zebra element, FTZ-F2 behaves as a transcriptional repressor of ftz. Point mutations in the zebra element which disrupt FTZ-F2 binding to DNA cause ectopic expression of zebra-lacZ activity in transformed embryos. The mutant constructs are expressed from the zygotic genome in preblastoderm embryos as early as the third nuclear division cycle. This unprecedented early transcription suggests that ftz requires active repression during initial nuclear division cycles, a novel type of embryonic gene regulation. A putative FTZ-F2 cDNA clone isolated by recognition site screening of an expression library was found to be identical in sequence with the zinc finger protein tramtrack (Harrison and Travers, 1990).
果蝇分节基因腹节基因在早期胚胎交替副节中的条纹状表达受740 bp斑马元件控制。在与斑马元件结合的多种蛋白质因子中，FTZ-F2作为ftz的转录抑制因子发挥作用。斑马元件中的点突变破坏了FTZ-F2与DNA的结合，导致转化胚胎中斑马-乳糖酶活性的异位表达。突变构建体早在第三次核分裂周期就在前胚盘胚胎的合子基因组中表达。这种前所未有的早期转录表明，ftz在最初的核分裂周期中需要活性抑制，这是一种新型的胚胎基因调控。通过对表达文库进行识别位点筛选分离得到的一个推定的FTZ-F2 cDNA克隆，其序列与锌指蛋白tramtrack相同（哈里森和特拉弗斯，1990年）。
