Division of Upper Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
J Gastroenterol. 2010 May;45(5):478-87. doi: 10.1007/s00535-009-0180-1. Epub 2009 Dec 18.
A traditional Japanese medicine, rikkunshito, has been reported to relieve dyspepsia symptoms. We investigated the effect of rikkunshito on RE-induced abdominal dyspepsia, and performed experiments to elucidate the mechanism of that effect.
RE model rats were prepared using 8-week-old male Wistar rats, and rikkunshito was administered in drinking water. Voluntary movement was used as an index of RE-induced abdominal dyspepsia, which was monitored by an infrared sensor. On the tenth day after surgery, the total area of esophageal erosion was measured, and samples of nonerosive mucosa were collected. Using those samples, intercellular spaces of epithelial mucosa were examined by transmission electron microscopy, and the NP-40-soluble and -insoluble levels of the tight junction proteins claudin-1, -3 and -4 and their mRNAs were determined.
Rikkunshito did not reduce the average total area of erosive lesions in the esophageal mucosa of RE model rats. On day 10, voluntary movement was significantly decreased in the RE model rats and rikkunshito significantly increased it. Nonerosive esophageal mucosa from RE rats showed dilation of intercellular spaces in epithelium, and significantly decreased claudin-3 mRNA and protein levels. Rikkunshito significantly suppressed intercellular space dilation and significantly increased the level of NP-40-insoluble claudin-3, but it did not affect the mRNA level, suggesting that it promoted tight junction formation by facilitating the translocation of proteins.
Rikkunshito increased voluntary movement in RE model rats. This may have been because rikkunshito ameliorated the symptoms of RE by improving the barrier function of esophageal mucosa.
一种传统的日本药物——六君子汤已被报道能缓解消化不良症状。我们研究了六君子汤对反流性食管炎（RE）引起的腹部消化不良的影响，并进行了实验以阐明其作用机制。
使用 8 周龄雄性 Wistar 大鼠制备 RE 模型大鼠，并在饮用水中给予六君子汤。通过红外传感器监测，以自愿运动作为 RE 引起的腹部消化不良的指标。手术后第 10 天，测量食管侵蚀的总区域，并采集非侵蚀性黏膜样本。使用这些样本，通过透射电子显微镜检查上皮黏膜的细胞间空间，并确定紧密连接蛋白 Claudin-1、-3 和 -4 的 NP-40 可溶性和不可溶性水平及其 mRNA。
六君子汤并未减少 RE 模型大鼠食管黏膜侵蚀性病变的平均总面积。在第 10 天，RE 模型大鼠的自愿运动明显减少，而六君子汤明显增加了它。RE 大鼠的非侵蚀性食管黏膜上皮细胞间空间扩张，Claudin-3mRNA 和蛋白水平明显降低。六君子汤显著抑制细胞间空间扩张，并显著增加 NP-40 不可溶性 Claudin-3 的水平，但不影响 mRNA 水平，表明它通过促进蛋白质转位促进紧密连接形成。
六君子汤增加了 RE 模型大鼠的自愿运动。这可能是因为六君子汤通过改善食管黏膜的屏障功能改善了 RE 的症状。
