Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Tamil Nadu, India.
Toxicol Mech Methods. 2007;17(1):33-40. doi: 10.1080/15376510600970026.
ABSTRACT The protective effect of dendrodoine analog (DA) [4-amino-5-benzoyl-2-(4-methoxy phenylamino) thiazole] at three doses (5, 10, and 15 mg/kg body weight) was investigated on ethanol-induced hyperlipidemia. Hepatotoxicity was induced by administering 7.9 g ethanol/kg body weight for 45 days by intragastric intubation. Our results showed increased activity of aspartate transaminase (AST), alkaline phosphatase (ALP), and gamma glutamyl transferase (GGT) and increased levels of cholesterol, triglycerides, and phospholipids in the plasma of alcohol-given group when compared with normal control group. The levels of tissue (liver and kidney) cholesterol and triglycerides were increased significantly in alcohol control rats when compared with normal control rats. The levels of phospholipids decreased significantly in the liver and kidney of alcohol control rats when compared with normal control rats. The activity of phospholipase A and phospholipase C increased significantly in the liver of alcohol control rats when compared with normal control rats. Intragastric administration of DA at 10 mg/kg body weight effectively lowered the activity of hepatic marker enzymes (GGT, AST, and ALP), phospholipase A, and phospholipase C, and decreased the levels of plasma and tissue lipids. The level of tissue phospholipids increased significantly when DA was administered at a dose of 10 mg/kg body weight along with alcohol when compared with alcohol control group. Thus, we propose that DA exerts a hepatoprotective effect by modulating liver marker enzymes and lipid levels at a dosage of 10 mg/kg body weight.
本研究旨在探讨树状多胺类似物（DA）[4-氨基-5-苯甲酰基-2-（4-甲氧基苯基氨基）噻唑]在三个剂量（5、10 和 15mg/kg 体重）下对乙醇诱导的高血脂的保护作用。通过灌胃给予 7.9g/kg 体重的乙醇，诱导 45 天的肝毒性。我们的结果表明，与正常对照组相比，给予乙醇的组的血浆中天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）和γ-谷氨酰转移酶（GGT）活性升高，胆固醇、甘油三酯和磷脂水平升高。与正常对照组相比，酒精对照组大鼠的组织（肝和肾）胆固醇和甘油三酯水平显著升高。与正常对照组相比，酒精对照组大鼠的肝和肾中磷脂水平显著降低。与正常对照组相比，酒精对照组大鼠肝中的磷脂酶 A 和磷脂酶 C 活性显著升高。给予 DA（10mg/kg 体重）可有效降低肝标志物酶（GGT、AST 和 ALP）、磷脂酶 A 和磷脂酶 C 的活性，并降低血浆和组织脂质水平。与酒精对照组相比，给予 DA（10mg/kg 体重）加酒精时组织磷脂水平显著升高。因此，我们提出 DA 通过调节肝标志物酶和脂质水平发挥肝保护作用，剂量为 10mg/kg 体重。
