La Nauze J M, Coggins J R, Dixon H B
Biochem J. 1977 Aug 1;165(2):409-11. doi: 10.1042/bj1650409.
Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), the bacterial enzyme that catalyses the reaction HCO-CH2-PO(OH)2+H2O leads to HCO-CH3+Pi, is inactivated by borohydride if either phosphonoacetaldehyde or acetaldehyde is present. This supports the suggestion that the substrate forms an imine with an amino group of the enzyme. Such imine formation would labilize the C-P bond in the same way that aldolase and related enzymes labilize C-C and C-H bonds (Scheme 1a).
膦酰乙醛水解酶（EC 3.11.1.1）是一种催化反应HCO-CH2-PO(OH)2 + H2O → HCO-CH3 + Pi的细菌酶，若存在膦酰乙醛或乙醛，它会被硼氢化物灭活。这支持了底物与酶的一个氨基形成亚胺的观点。这种亚胺的形成会使C-P键不稳定，其方式与醛缩酶及相关酶使C-C和C-H键不稳定的方式相同（方案1a）。
