School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2613-2617. doi: 10.1099/ijs.0.019463-0. Epub 2009 Dec 18.
Two halophilic archaea, strains RO1-4(T) and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4(T) and RO1-64 were able to grow at 25-55 °C (optimum 40-42 °C), at 2.1-5.1 M NaCl (optimum 3.9 M NaCl), at 0.05-0.7 M MgCl(2) (optimum 0.3 M MgCl(2)) and at pH 6.0-8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4(T) and RO1-64 were closely related to Halogeometricum borinquense PR3(T) (98.0 and 98.2 % similarity, respectively) and Halosarcina pallida BZ256(T) (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4(T) and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA-DNA hybridization value between strains RO1-4(T) and RO1-64 was 83.0 % and both strains showed low DNA-DNA relatedness with Halogeometricum borinquense PR3(T) (42.5 and 50.1 % relatedness, respectively) and Halosarcina pallida BZ256(T) (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4(T) and RO1-64 represent a novel species of the genus Halogeometricum, for which the name Halogeometricum rufum sp. nov. is proposed. The type strain is RO1-4(T) (=CGMCC 1.7736(T) =JCM 15770(T)).
两株嗜盐古菌，RO1-4(T) 和 RO1-64 株，从中国江苏的一个海洋晒盐场分离得到。这两株菌的细胞形态多形，运动，革兰氏染色阴性。菌落呈红色。RO1-4(T) 和 RO1-64 株可在 25-55°C（最佳 40-42°C）、2.1-5.1 M NaCl（最佳 3.9 M NaCl）、0.05-0.7 M MgCl2（最佳 0.3 M MgCl2）和 pH 6.0-8.5（最佳 pH 7.0）下生长。细胞在蒸馏水中溶解，防止细胞溶解的最小 NaCl 浓度为 12%（w/v）。基于 16S rRNA 基因序列分析，RO1-4(T) 和 RO1-64 株与 Halogeometricum borinquense PR3(T)（分别为 98.0%和 98.2%相似）和 Halosarcina pallida BZ256(T)（分别为 97.8%和 97.9%相似）密切相关。这两株菌的主要极性脂是磷脂酰甘油、磷脂酰甘油磷酸甲基酯和两种糖脂（S-DGD-1 和 DGD-1）。RO1-4(T) 和 RO1-64 株的 DNA G+C 含量分别为 64.9mol%和 62.4mol%。RO1-4(T) 和 RO1-64 株之间的 DNA-DNA 杂交值为 83.0%，与 Halogeometricum borinquense PR3(T)（分别为 42.5%和 50.1%的相关度）和 Halosarcina pallida BZ256(T)（分别为 37.6%和 42.1%的相关度）的 DNA-DNA 相关度较低。结论是，RO1-4(T) 和 RO1-64 株代表 Halogeometricum 属的一个新种，命名为 Halogeometricum rufum sp. nov.。模式菌株为 RO1-4(T)（=CGMCC 1.7736(T)=JCM 15770(T)）。
