School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2010 Sep;60(Pt 9):2089-2093. doi: 10.1099/ijs.0.018598-0. Epub 2009 Oct 23.
Two extremely halophilic archaea, strains RO5-2(T) and RO5-14, were isolated from Rudong marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile and stained Gram-negative. Colonies were red-pigmented. Strains RO5-2(T) and RO5-14 were able to grow at 20-50 degrees C (optimum 37 degrees C), at 2.6-4.8 M NaCl (optimum 3.4-3.9 M NaCl), at 0.03-0.7 M MgCl(2) (optimum 0.5 M MgCl(2)) and at pH 5.5-8.0 (optimum pH 6.5-7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene sequences of strains RO5-2(T) and RO5-14 showed 93.4-93.8 % similarity to the closest cultivated relative, Halosarcina pallida. The DNA G+C content of strains RO5-2(T) and RO5-14 was 61.0 mol% and 59.9 mol%, respectively. The DNA-DNA relatedness between strains RO5-2(T) and RO5-14 was 86.0 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains RO5-2(T) and RO5-14 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halopelagius inordinatus gen. nov., sp. nov. is proposed. The type strain is RO5-2(T) (=CGMCC 1.7739(T) =JCM 15773(T)).
两株极端嗜盐古菌 RO5-2(T) 和 RO5-14 从中国江苏如东海洋晒盐场中分离得到。两株菌的细胞呈多形性，运动性且革兰氏染色阴性。菌落呈红色。RO5-2(T) 和 RO5-14 株在 20-50°C（最适 37°C）、2.6-4.8 M NaCl（最适 3.4-3.9 M NaCl）、0.03-0.7 M MgCl2（最适 0.5 M MgCl2）和 pH5.5-8.0（最适 pH6.5-7.0）下生长。细胞在蒸馏水中溶解，防止细胞溶解的最小 NaCl 浓度为 12%（w/v）。两株菌的主要极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲基酯和两种主要糖脂，与硫酸甘露糖葡糖基二醚（S-DGD-1）和甘露糖葡糖基二醚（DGD-1）在色谱上相同。RO5-2(T) 和 RO5-14 株的 16S rRNA 基因序列与最接近的培养相关种 Halosarcina pallida 的相似性为 93.4-93.8%。RO5-2(T) 和 RO5-14 株的 DNA G+C 含量分别为 61.0 mol%和 59.9 mol%。RO5-2(T) 和 RO5-14 株之间的 DNA-DNA 相关性为 86.0%。表型、化学分类和系统发育特性表明，RO5-2(T) 和 RO5-14 株代表了 Halobacteriaceae 科一个新属中的一个新种，命名为 Halopelagius inordinatus gen. nov.，sp. nov.。模式株为 RO5-2(T)（=CGMCC 1.7739(T)=JCM 15773(T)）。
