将高效的 T7 表达系统改编为链霉菌属。
Adaptation of the highly productive T7 expression system to Streptomyces lividans.
机构信息
INRS-Institut Armand-Frappier, 531 Boul. des Prairies, Laval, Québec, Canada H7V 1B7.
出版信息
Appl Environ Microbiol. 2010 Feb;76(3):967-70. doi: 10.1128/AEM.02186-09. Epub 2009 Dec 18.
Abstract
Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.
摘要
链霉菌是一种革兰氏阳性菌，以其出色的分泌效率和低细胞外蛋白酶活性而闻名。在本工作中，我们将高效的 T7 表达系统应用于链霉菌。通过染色体整合的方式实现了对优化后的 T7 RNA 聚合酶基因的表达，并构建了一种双功能 T7 表达载体。
相似文献
1
Adaptation of the highly productive T7 expression system to Streptomyces lividans.
Appl Environ Microbiol. 2010 Feb;76(3):967-70. doi: 10.1128/AEM.02186-09. Epub 2009 Dec 18.
2
Identification of the first functional toxin-antitoxin system in Streptomyces.
PLoS One. 2012;7(3):e32977. doi: 10.1371/journal.pone.0032977. Epub 2012 Mar 14.
3
S-Adenosylmethionine induces BldH and activates secondary metabolism by involving the TTA-codon control of bldH expression in Streptomyces lividans.
Arch Microbiol. 2008 Apr;189(4):419-26. doi: 10.1007/s00203-007-0336-4. Epub 2007 Dec 14.
4
Development of nitrilase promoter-derived inducible vectors for Streptomyces.
Biosci Biotechnol Biochem. 2016 Jun;80(6):1230-7. doi: 10.1080/09168451.2016.1148577. Epub 2016 Feb 29.
5
Chromogenic identification of promoters in Streptomyces lividans by using an ampC beta-lactamase promoter-probe vector.
Mol Gen Genet. 1987 Nov;210(1):23-32. doi: 10.1007/BF00337754.
6
Heterologous expression of human interleukin-6 in Streptomyces lividans TK24 using novel secretory expression vectors.
Biotechnol Lett. 2011 Feb;33(2):253-61. doi: 10.1007/s10529-010-0428-0. Epub 2010 Oct 8.
7
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production.
Methods Mol Biol. 2023;2555:213-260. doi: 10.1007/978-1-0716-2795-2_16.
8
High-level overproduction of Thermus enzymes in Streptomyces lividans.
Appl Microbiol Biotechnol. 2008 Jul;79(6):1001-8. doi: 10.1007/s00253-008-1495-1. Epub 2008 May 7.
9
Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans.
Biotechnol Lett. 2011 Apr;33(4):727-31. doi: 10.1007/s10529-010-0480-9. Epub 2010 Nov 30.
10
Fate of the sblA transcript in Streptomyces lividans and Escherichia coli.
FEMS Microbiol Lett. 2007 Nov;276(1):42-7. doi: 10.1111/j.1574-6968.2007.00912.x. Epub 2007 Sep 17.
引用本文的文献
1
A precisely regulated Streptomyces expression system pTZYp facilitated by the strong promoter stnY and cmt operon.
Biotechnol Lett. 2025 Aug 12;47(5):89. doi: 10.1007/s10529-025-03628-x.
2
Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits.
Nat Commun. 2025 Jan 17;16(1):774. doi: 10.1038/s41467-025-55973-0.
3
A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.
Bioresour Bioprocess. 2022 May 18;9(1):56. doi: 10.1186/s40643-022-00540-4.
4
CRISPR-aided genome engineering for secondary metabolite biosynthesis in Streptomyces.
J Ind Microbiol Biotechnol. 2024 Jan 9;51. doi: 10.1093/jimb/kuae009.
5
Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes.
Appl Microbiol Biotechnol. 2024 Jan 30;108(1):185. doi: 10.1007/s00253-023-12900-x.
6
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production.
Methods Mol Biol. 2023;2555:213-260. doi: 10.1007/978-1-0716-2795-2_16.
7
T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation.
Microb Cell Fact. 2022 Jan 28;21(1):13. doi: 10.1186/s12934-022-01740-5.
8
as Microbial Chassis for Heterologous Protein Expression.
Front Bioeng Biotechnol. 2021 Dec 21;9:804295. doi: 10.3389/fbioe.2021.804295. eCollection 2021.
9
T7 RNA polymerase-driven inducible cell lysis for DNA transfer from Escherichia coli to Bacillus subtilis.
Microb Biotechnol. 2017 Nov;10(6):1797-1808. doi: 10.1111/1751-7915.12843. Epub 2017 Aug 16.
10
Engineering microbial hosts for production of bacterial natural products.
Nat Prod Rep. 2016 Aug 27;33(8):963-87. doi: 10.1039/c6np00017g. Epub 2016 Apr 13.
本文引用的文献
1
A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatus.
Protein Expr Purif. 2010 Feb;69(2):137-46. doi: 10.1016/j.pep.2009.08.008. Epub 2009 Aug 23.
2
A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium.
Appl Microbiol Biotechnol. 2009 Apr;82(6):1195-203. doi: 10.1007/s00253-009-1952-5. Epub 2009 Mar 24.
3
Extracellular signalling, translational control, two repressors and an activator all contribute to the regulation of methylenomycin production in Streptomyces coelicolor.
Mol Microbiol. 2009 Feb;71(3):763-78. doi: 10.1111/j.1365-2958.2008.06560.x. Epub 2008 Dec 1.
4
Homologous cloning, expression, and characterisation of a laccase from Streptomyces coelicolor and enzymatic decolourisation of an indigo dye.
Appl Microbiol Biotechnol. 2008 Jun;79(4):597-603. doi: 10.1007/s00253-008-1475-5. Epub 2008 Apr 24.
5
The use of the rare UUA codon to define "expression space" for genes involved in secondary metabolism, development and environmental adaptation in streptomyces.
J Microbiol. 2008 Feb;46(1):1-11. doi: 10.1007/s12275-007-0233-1.
6
One step engineering of T7-expression strains for protein production: increasing the host-range of the T7-expression system.
Protein Expr Purif. 2007 Oct;55(2):325-33. doi: 10.1016/j.pep.2007.06.014. Epub 2007 Jul 12.
7
Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems.
Appl Microbiol Biotechnol. 2006 Sep;72(2):211-22. doi: 10.1007/s00253-006-0465-8. Epub 2006 Jun 22.
8
Streptomyces inside-out: a new perspective on the bacteria that provide us with antibiotics.
Philos Trans R Soc Lond B Biol Sci. 2006 May 29;361(1469):761-8. doi: 10.1098/rstb.2005.1758.
9
Expression of the regulatory protein LndI for landomycin E production in Streptomyces globisporus 1912 is controlled by the availability of tRNA for the rare UUA codon.
FEMS Microbiol Lett. 2006 Mar;256(1):30-7. doi: 10.1111/j.1574-6968.2005.00087.x.
10
Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity.
Protein Expr Purif. 2006 Apr;46(2):274-84. doi: 10.1016/j.pep.2005.09.008. Epub 2005 Oct 6.
Zotero 插件