Zhang Mingzi M, Wang Yajie, Ang Ee Lui, Zhao Huimin
Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research, Singapore.
Nat Prod Rep. 2016 Aug 27;33(8):963-87. doi: 10.1039/c6np00017g. Epub 2016 Apr 13.
Covering up to end 2015Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.
涵盖至2015年底
微生物发酵为结构复杂的天然产物生产提供了一种有吸引力的化学合成替代方法。然而,在大多数情况下,生产滴度较低,需要提高以用于化合物表征和/或商业生产。由于功能基因组学和基因工程技术的进步,可以对微生物宿主进行工程改造以过量生产所需的天然产物,极大地加速了传统上耗时的菌株改良过程。本综述涵盖了用于生产细菌天然产物的天然和异源微生物宿主工程的最新进展和挑战,重点是菌株改良的遗传工具和策略。特别强调了放线菌产生的生物活性次级代谢产物。本综述还将讨论宿主系统选择的考虑因素。