Department of Chemistry, University of Rome La Sapienza, P. le Aldo Moro, 5-00185 Roma, Italy.
Phys Chem Chem Phys. 2010 Jan 14;12(2):516-22. doi: 10.1039/b911433e. Epub 2009 Nov 17.
Water dynamics and compartmentation in glutaraldehyde cross-linked bovine serum albumin nanoparticles have been investigated by an integrated nuclear magnetic resonance (NMR) protocol based on water relaxation times and self-diffusion coefficients measurements. Multi-exponentially of water relaxation curves has been accounted for according to a diffusive and chemical exchange model (see B. P. Hills, S. F. Takacs and P. S. Belton, Mol. Phys., 1989, 67(4), 903, and Mol. Phys., 1989, 67(4), 913; E. Brosio, M. Belotti and R. Gianferri, in Food Science and Technology: New Research, ed. L. V. Greco and M. N. Bruno, Nova Science Publishers, Hauppauge (NY), 2008) that made it possible to single out water molecules in the molecular spaces in the interior of albumin nanoparticles, in the meso-cavities formed by packed nanoparticles and in the meniscus on top of the nanoparticles suspension. A quantitative rationalization of T(2) values of water different components allowed morphological information to be acquired as for the size of water filled compartments, while self-diffusion coefficient measurements of water excess or fluxed packed nanoparticles suspensions are describers of transport properties of soft biomaterials. The paper reports an NMR approach that can be seen as a general and relevant method to characterize excess-water-swollen soft biomaterials.
利用基于水弛豫时间和自扩散系数测量的综合核磁共振（NMR）协议，研究了戊二醛交联牛血清白蛋白纳米颗粒中的水动力学和隔室化。根据扩散和化学交换模型（见 B.P. Hills、S.F. Takacs 和 P.S. Belton，Mol. Phys.，1989，67（4），903，和 Mol. Phys.，1989，67（4），913；E. Brosio、M. Belotti 和 R. Gianferri，in Food Science and Technology: New Research，ed. L.V. Greco 和 M.N. Bruno，Nova Science Publishers，Hauppauge (NY)，2008），对多指数水弛豫曲线进行了处理，从而可以分离出白蛋白纳米颗粒内部分子空间、由纳米颗粒堆积形成的中间空腔以及纳米颗粒悬浮液顶部的弯月面中的水分子。对不同水成分的 T（2）值进行定量合理化处理，可以获得关于充满水隔室的大小等形态信息，而水过量或通量填充的纳米颗粒悬浮液的自扩散系数测量则描述了软生物材料的传输特性。本文报道了一种 NMR 方法，可视为一种通用且相关的方法，用于表征过湿软生物材料。
