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基于谷胱甘肽的金纳米簇用于选择性结合和检测细胞裂解物中的谷胱甘肽 S-转移酶融合蛋白。

Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates.

机构信息

Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.

出版信息

Chem Commun (Camb). 2009 Dec 28(48):7515-7. doi: 10.1039/b916919a. Epub 2009 Nov 3.

DOI:10.1039/b916919a
PMID:20024264
Abstract

A straightforward method for the rapid detection of the presence of glutathione S-transferase (GST)-tagged proteins from sample solutions using glutathione (GSH)-bound gold nanoclusters (Au@GSH NCs) with luminescence properties as the detection probes by simple observation with the naked eye was proposed in this study.

摘要

本研究提出了一种简便的方法，使用具有发光性质的谷胱甘肽（GSH）结合的金纳米簇（Au@GSH NCs）作为检测探针，通过简单的肉眼观察，从样品溶液中快速检测谷胱甘肽 S-转移酶（GST）标记蛋白的存在。

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