Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
Nanoscale Res Lett. 2013 Apr 15;8(1):170. doi: 10.1186/1556-276X-8-170.
Biomimetic synthesis has become a promising green pathway to prepare nanomaterials. In this study, bovine serum albumin (BSA)-conjugated gold nanoclusters/nanoparticles were successfully synthesized in water at room temperature by a protein-directed, solution-phase, green synthetic method. The synthesized BSA-Au nanocomplexes have fluorescence emission (588 nm) of gold nanoclusters and surface plasmon resonance of gold nanoparticles. The BSA-Au nanocomplexes display non-cytotoxicity and excellent biocompatibility on MGC803 gastric cancer cells. After conjugation of folic acid molecules, the obtained BSA-Au nanocomplexes showed highly selective targeting for MGC803 cells and dual-modality dark-field and fluorescence imaging.
仿生合成已成为一种有前途的绿色途径来制备纳米材料。在这项研究中，通过一种蛋白质导向的、溶液相的、绿色合成方法，在水相中于室温下成功合成了牛血清白蛋白（BSA）- 共轭金纳米团簇/纳米粒子。合成的 BSA-Au 纳米复合物具有金纳米团簇的荧光发射（588nm）和金纳米粒子的表面等离子体共振。BSA-Au 纳米复合物在 MGC803 胃癌细胞上表现出非细胞毒性和优异的生物相容性。在接枝叶酸分子后，所得到的 BSA-Au 纳米复合物对 MGC803 细胞表现出高度选择性的靶向作用，并具有暗场和荧光双模成像。
