在一株对替加环素耐药的肠炎沙门氏菌哈达尔血清型临床分离株中发现了 ramR 突变和 Tn1721 相关 tet(A)变体的存在。
Combined ramR mutation and presence of a Tn1721-associated tet(A) variant in a clinical isolate of Salmonella enterica serovar Hadar resistant to tigecycline.
机构信息
Institut für Medizinische Mikrobiologie, Virologie und Hygiene, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
出版信息
Antimicrob Agents Chemother. 2010 Mar;54(3):1319-22. doi: 10.1128/AAC.00993-09. Epub 2009 Dec 22.
Abstract
A Salmonella enterica serovar Hadar strain resistant to tigecycline (MIC, 16 microg/ml) was isolated. Molecular characterization revealed the presence of a plasmid-borne tet(A) variant associated with Tn1721 mediating a rise of the MIC for tigecycline when transferred to Escherichia coli. Additionally, a truncating mutation in ramR was detected. Transformation with wild-type ramR but not with the mutated ramR lowered the MIC for tigecycline. Characterization of this Salmonella isolate implicates ramR in resistance to tigecycline.
摘要
一株对替加环素（MIC，16μg/ml）耐药的肠炎沙门氏菌哈达尔（Salmonella enterica serovar Hadar）菌株被分离出来。分子特征分析显示存在一个与 Tn1721 相关的可移动质粒 tet(A)变体，该变体介导替加环素 MIC 升高，当转移到大肠杆菌时会出现这种情况。此外，还检测到 ramR 中的截断突变。用野生型 ramR 转化，但不用突变型 ramR 转化，会降低替加环素的 MIC。对这种沙门氏菌分离株的特征分析表明，ramR 与对替加环素的耐药性有关。
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