Department of Nuclear Medicine, Chonnam National University Medical School, Dong-gu, Gwangju, Republic of Korea.
Cancer Res. 2010 Jan 1;70(1):18-23. doi: 10.1158/0008-5472.CAN-09-3453. Epub 2009 Dec 22.
Tumor-targeting bacteria have been studied in terms of their ability to visualize the infection pathway (through imaging probes) or to carry therapeutic molecules to tumors. To integrate these monitoring and therapeutic functions, we engineered attenuated Salmonella typhimurium defective in guanosine 5'-diphosphate-3'-diphosphate synthesis to carry cytotoxic proteins (cytolysin A) and express reporter genes. We successfully visualized the therapeutic process with these engineered bacteria in mice and found that they often mediated complete tumor (CT-26) eradication on cytotoxic gene induction. Furthermore, treatment with the engineered bacteria markedly suppressed metastatic tumor growth.
已经研究了靶向肿瘤的细菌，以评估其可视化感染途径（通过成像探针）或向肿瘤输送治疗分子的能力。为了整合这些监测和治疗功能，我们构建了缺失鸟苷 5′-二磷酸-3′-二磷酸合成的减毒鼠伤寒沙门氏菌，使其携带细胞毒性蛋白（细胞溶素 A）并表达报告基因。我们成功地在小鼠中用这些工程细菌可视化了治疗过程，并发现它们在诱导细胞毒性基因时经常介导完全的肿瘤（CT-26）清除。此外，用工程细菌治疗明显抑制了转移性肿瘤的生长。
