Service of Diabetes, Endocrinology and Nutrition, Institut d'Investigació Biomèdica de Girona, CIBEROBN (CB06/03/0010) and Instituto de Salud Carlos III, Girona, Spain.
Int J Obes (Lond). 2010 Mar;34(3):487-99. doi: 10.1038/ijo.2009.263. Epub 2009 Dec 22.
Very limited information is available regarding the function of human thyroid hormone responsive Spot 14 (human S14, hS14) in adipogenesis and human adiposity.
To evaluate hS14 levels during differentiation of human pre-adipocytes, in human fat depots and isolated fat cells.
This was a cross-sectional study.
A total of 161 omental (OM) and 87 subcutaneous (SC) adipose tissue samples obtained during elective surgical procedures from a population who varied widely in terms of obesity.
hS14 gene expression and protein levels during adipogenesis were assessed by RT-PCR, western blot, and using an automated confocal imaging approach.
hS14 gene expression levels were decreased in OM adipose tissue from overweight (-42.0%) and obese subjects (-56.5%) compared with lean subjects (P<0.05 and P<0.0001, respectively). hS14 mRNA (but not hS14-related) was inversely associated with obesity measures such as body mass index (P=0.001), percent fat mass (P=0.001), waist-to-hip ratio (P=0.020), and systolic blood pressure (P=0.031). hS14 gene expression and protein levels were up-regulated at the early stages of differentiation of human pre-adipocytes as well as for 3T3-L1 cells. That observation was most prominent in those individual cells exhibiting the more marked differentiation features. hS14 gene expression levels increased by approximately 45 000-fold in mature adipocytes. Increased hS14 levels were also found in stromal-vascular cells/pre-adipocytes (3.8-fold, P<0.05) and in adipose tissue samples (1.9-fold, P<0.0001) from SC compared with OM fat depots.
These results suggest that hS14 is involved in human adipogenesis, but inversely related to obesity and OM fat accumulation.
关于人类甲状腺激素反应性 Spot 14（人类 S14，hS14）在脂肪生成和人类肥胖中的功能，信息非常有限。
评估人前脂肪细胞分化过程中、人体脂肪组织和分离脂肪细胞中 hS14 的水平。
这是一项横断面研究。
从不同肥胖程度的人群接受择期手术时获得的总共 161 个网膜（OM）和 87 个皮下（SC）脂肪组织样本。
通过 RT-PCR、western blot 和自动共聚焦成像方法评估脂肪生成过程中 hS14 基因表达和蛋白水平。
与瘦受试者相比，超重（-42.0%）和肥胖（-56.5%）受试者的 OM 脂肪组织中 hS14 基因表达水平降低（P<0.05 和 P<0.0001）。hS14 mRNA（而非 hS14 相关）与肥胖指标如体重指数（P=0.001）、体脂肪百分比（P=0.001）、腰臀比（P=0.020）和收缩压（P=0.031）呈负相关。人前脂肪细胞的早期分化以及 3T3-L1 细胞中 hS14 基因表达和蛋白水平上调。在表现出更明显分化特征的个别细胞中，这种观察最为明显。成熟脂肪细胞中 hS14 基因表达水平增加了约 45000 倍。与 OM 脂肪组织相比，SC 中的基质血管细胞/前脂肪细胞（3.8 倍，P<0.05）和脂肪组织样本（1.9 倍，P<0.0001）中也发现 hS14 水平升高。
这些结果表明 hS14 参与了人类脂肪生成，但与肥胖和 OM 脂肪堆积呈负相关。
