Service of Diabetes, Endocrinology and Nutrition (UDEN), Institut d'Investigació Biomèdica de Girona, CIBER de Fisiopatología de Obesidad y Nutrición (CIBERobn, CB06/03/0010), and Instituto de Salud Carlos III, Girona, Spain.
Int J Obes (Lond). 2012 Feb;36(2):320-4. doi: 10.1038/ijo.2011.101. Epub 2011 May 24.
Differentiation and metabolism of adipose tissue are modulated by thyroid hormones (THs), but relatively little is known about the metabolism of THs in this tissue. Expression of the genes for type I iodothyronine 5'-deiodinase (D1), leptin (LEP) and stearoyl-CoA desaturase 1 (SCD-1) was evaluated in omental (OM) and subcutaneous (SC) fat using a cohort of 70 humans. Activities of iodothyronine deiodinases (D1, D2 and D3) were assessed in a randomly selected subpopulation of 19 subjects. D1 expression was upregulated in both OM (P=0.011) and SC (P=0.003) fat of obese subjects. Concomitantly, OM (P=0.002) and SC (P=0.028) LEP expression were increased in obesity, associated with both D1 mRNA (r=0.315, P=0.014) and activity (r=0.647, P=0.023) and inversely related to SCD-1 (r=-0.266, P=0.034) expression in SC fat. Also D1 (but not D2 and D3) activity was increased in OM (∼fourfold, P=0.010) and SC (∼eightfold, P=0.004) fat of obese when compared with non-obese subjects and correlated in both OM (r=0.528, P=0.036) and SC (r=0.749, P=0.005) fat with body mass index. Our results document increased D1 gene expression and activity in adipose tissue of obese humans and suggest a role of 3,5,3'-triiodo-L-thyronine formed by D1 in response to leptin in the modulation of adipose tissue metabolism.
脂肪组织的分化和代谢受甲状腺激素（THs）调节，但人们对该组织中 THs 的代谢知之甚少。本研究使用 70 名人类的队列评估了腹内（OM）和皮下（SC）脂肪中 I 型碘甲状腺原氨酸 5'-脱碘酶（D1）、瘦素（LEP）和硬脂酰辅酶 A 去饱和酶 1（SCD-1）基因的表达。在随机选择的 19 名受试者亚群中评估了碘甲状腺原氨酸脱碘酶（D1、D2 和 D3）的活性。在肥胖者的 OM（P=0.011）和 SC（P=0.003）脂肪中，D1 表达上调。同时，肥胖者 OM（P=0.002）和 SC（P=0.028）LEP 表达增加，与 D1 mRNA（r=0.315，P=0.014）和活性（r=0.647，P=0.023）呈正相关，与 SC 脂肪中的 SCD-1 表达呈负相关（r=-0.266，P=0.034）。此外，与非肥胖者相比，肥胖者 OM（增加约 4 倍，P=0.010）和 SC（增加约 8 倍，P=0.004）脂肪中的 D1（但不是 D2 和 D3）活性增加，并与 OM（r=0.528，P=0.036）和 SC（r=0.749，P=0.005）脂肪中的体重指数相关。我们的研究结果记录了肥胖人群脂肪组织中 D1 基因表达和活性增加，并表明 D1 形成的 3,5,3'-三碘-L-甲状腺素在响应瘦素调节脂肪组织代谢中的作用。
