Gharipour Mojgan, Sadeghi Masoumeh, Behmanesh Mehrdad, Salehi Mansour, Roohafza Hamidreza, Nezafati Pouya, Khosravi Elham, Hosseini Mohsen, Keshvari Mahtab, Rouhi-Bourojeni Hojat, Sarrafzadegan Nizal
Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Acta Biomed. 2019 Jan 22;90(1):44-50. doi: 10.23750/abm.v90i1.6167.
Selenium is the component of selenocystein amino acid, which itself is the building block of selenoproteins having diverse effects on various aspects of the human health. Among these proteins, selenoprotein P is the central to the distribution and homeostasis of selenium, and selenoprotein S as a transmembrane protein is associated with a range of inflammatory markers, particularly in the context of cardiovascular disease. It is known that selenium status outside of the normal range is considered to confer different benefits or adverse cardiovascular risk factors. Therefore, for the first time, we aimed to verify effects of Selenium supplementation on Selenoprotein P and S Genes Expression in Protein and mRNA Levels in Subjects with Coronary Artery Disease (CAD).
This is the study protocol of a double blinded randomized clinical trial on 130 subjects with angiographically documented stenosis of more than 75% in one or more coronary artery vessels. In this 60-day study, 65 patients in each group received either a 200mg selenium yeast or placebo tablets once daily. During the study, subjects were followed by phone calls and visited our clinic twice to repeat baseline measurements. We hypothesized that our finding would enable a more basic and confirmed understanding for the effect of selenium supplementation by investigating its effect on gene expression levels in people with CAD.
Upon confirmation of this hypothesis, the beneficial effect of inflammation regulation by supplementation with micronutrients could be considered for subjects with CVD.
硒是硒代半胱氨酸氨基酸的组成部分，而硒代半胱氨酸本身是硒蛋白的构建基块，对人体健康的各个方面具有多种影响。在这些蛋白质中，硒蛋白P对于硒的分布和稳态至关重要，而硒蛋白S作为一种跨膜蛋白与一系列炎症标志物相关，特别是在心血管疾病的背景下。已知超出正常范围的硒状态被认为会带来不同的益处或不利的心血管危险因素。因此，我们首次旨在验证补充硒对冠状动脉疾病（CAD）患者硒蛋白P和S基因在蛋白质和mRNA水平表达的影响。
这是一项针对130名经血管造影证实一根或多根冠状动脉血管狭窄超过75%的受试者的双盲随机临床试验的研究方案。在这项为期60天的研究中，每组65名患者每天服用一次200毫克硒酵母或安慰剂片。在研究期间，通过电话随访受试者，并让他们到我们的诊所就诊两次以重复基线测量。我们假设我们的发现将通过研究其对CAD患者基因表达水平的影响，从而对补充硒的效果有更基本和确定的理解。
在证实这一假设后，对于患有心血管疾病的受试者，可以考虑补充微量营养素对炎症调节的有益作用。
