巴基斯坦人群中肿瘤坏死因子α基因多态性G-308A与假性剥脱性青光眼的关联
Association of tumor necrosis factor alpha gene polymorphism G-308A with pseudoexfoliative glaucoma in the Pakistani population.
作者信息
Khan Muhammad Imran, Micheal Shazia, Rana Noreen, Akhtar Farah, den Hollander Anneke I, Ahmed Asifa, Qamar Raheel
机构信息
Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan.
出版信息
Mol Vis. 2009 Dec 22;15:2861-7.
PURPOSE
The purpose of the present study was to determine the role of the tumor necrosis factor alpha (TNF-alpha) gene polymorphism G-308A and total serum immunoglobulin E (TsIgE) levels in the onset of pseudoexfoliation glaucoma (PEXG) in Pakistani patients.
METHODS
The TNF-alpha polymorphism G-308A was analyzed in 122 patients with PEXG and 126 healthy unrelated controls by using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). TsIgE levels were determined by solid-phase enzyme-linked immunosorbent assay (ELISA).
RESULTS
The AA and GA genotypes were strongly associated with PEXG (p<0.001), with an odds ratio (OR) of 0.07 (95% confidence interval [CI]=0.02-0.27) and 0.24 (95% CI=0.12-0.51), respectively, while the GG genotype was found at a higher frequency in controls as compared to patients (p<0.001) OR=8.95 (95% CI=4.55-17.81). No significant difference was found in TsIgE levels of both patients and controls (p=0.86).
CONCLUSION
The present study concludes that the TNF-alpha polymorphism G-308A is strongly associated with PEXG. To our knowledge this is the first study in southeast Asia which demonstrates a strong association of a TNF-alpha polymorphism with PEXG.
目的
本研究旨在确定肿瘤坏死因子α（TNF-α）基因多态性G-308A和血清总免疫球蛋白E（TsIgE）水平在巴基斯坦患者假性剥脱性青光眼（PEXG）发病中的作用。
方法
采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析122例PEXG患者和126例健康无关对照者的TNF-α基因多态性G-308A。通过固相酶联免疫吸附测定（ELISA）测定TsIgE水平。
结果
AA和GA基因型与PEXG密切相关（p<0.001），优势比（OR）分别为0.07（95%置信区间[CI]=0.02-0.27）和0.24（95%CI=0.12-0.51），而GG基因型在对照组中的频率高于患者组（p<0.001），OR=8.95（95%CI=4.55-17.81）。患者和对照组的TsIgE水平无显著差异（p=0.86）。
结论
本研究得出结论，TNF-α基因多态性G-308A与PEXG密切相关。据我们所知，这是东南亚地区第一项证明TNF-α基因多态性与PEXG有强关联的研究。
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