Department of General Surgery, Gaziantep University School of Medicine, Turkey.
Cell Biochem Funct. 2010 Jan;28(1):83-8. doi: 10.1002/cbf.1624.
Nigella sativa (NS) has been shown to have antioxidant and antiinflammatory activities in different conditions. The goal of this study was to evaluate the effects of NS on cholestatic liver injury in rats. Thirty rats were recruited in the study as follows: Group 1, Bile duct ligation (BDL) (n = 10); Group 2, BDL plus NS (n = 10); and Group 3, Sham (n = 10). Bile duct ligated group received 0.2 mL kg(-1) dose of NS intraperitoneally daily throughout 14 days. Liver damage and cholestasis were determined by the biochemical and the pathologic examination. Data showed a decrease in gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) activities of the NS treated rats when compared with BDL group (p < 0.001 for GGT and p < 0.05 for others). The NS treated rats' tissue levels of total oxidant status (TOS), oxidative stress index (OSI), and myeloperoxidase (MPO) were significantly lower than that of the BDL group (p < 0.01 for all). Increases in total antioxidant capacity (TAC) and catalase (CAT) levels were statistically significant in the NS treated rats compared to BDL group (p < 0.01 for both). On the other hand, administration of NS in the rats with biliary obstruction resulted in inhibition of necro-inflammation. These results indicate that NS exerts a therapeutic effect on cholestatic liver injury in bile duct ligated rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.
黑种草（NS）已被证明在不同条件下具有抗氧化和抗炎活性。本研究的目的是评估 NS 对胆管结扎大鼠胆汁淤积性肝损伤的影响。30 只大鼠被纳入研究，如下所示：第 1 组，胆管结扎（BDL）（n = 10）；第 2 组，BDL 加 NS（n = 10）；第 3 组，假手术（Sham）（n = 10）。胆管结扎组在 14 天内每天腹腔内给予 0.2 mL kg(-1)剂量的 NS。通过生化和病理检查确定肝损伤和胆汁淤积。数据显示，与 BDL 组相比，NS 治疗大鼠的γ谷氨酰转移酶（GGT）、碱性磷酸酶（ALP）、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和乳酸脱氢酶（LDH）活性降低（GGT 为 p < 0.001，其他为 p < 0.05）。NS 治疗大鼠的总氧化剂状态（TOS）、氧化应激指数（OSI）和髓过氧化物酶（MPO）的组织水平明显低于 BDL 组（均为 p < 0.01）。与 BDL 组相比，NS 治疗大鼠的总抗氧化能力（TAC）和过氧化氢酶（CAT）水平显著升高（均为 p < 0.01）。另一方面，在胆管阻塞的大鼠中给予 NS 导致坏死性炎症的抑制。这些结果表明，NS 通过抑制肝组织中中性粒细胞浸润和氧化应激，对胆管结扎大鼠的胆汁淤积性肝损伤发挥治疗作用。
