Parhizkar Saadat, Latiff Latiffah A
Medicinal Plants Research Centre, Yasuj University of Medical Sciences (YUMS),Yasuj, Iran.
Adv Pharm Bull. 2013;3(1):31-6. doi: 10.5681/apb.2013.006. Epub 2013 Feb 7.
This study aimed to evaluate the possible estrogenic activity of some ingredients of Nigella sativa including Linoleic acid and Gama-Linolenic acid by vaginal cornification assay.
Forty ovariectomized (OVX) rats, aged 16 weeks were allotted randomly to five groups: negative control (taking 1 ml olive oil/ day); positive control (taking 0.2 mg/kg/day Conjucated Equine Estrogen-CEE); experimental groups (taking 50 mg/kg/day Linoleic acid or 10 mg/kg/day Gamma Linolenic acid or 15mg/kg/day Thymoquinone ). All of supplements administered via intragastric gavage for 21 consecutive days. To assess estrogen like activity, vaginal smear was examined daily and serum estradiol was measured at baseline, after 10 days and at the end of experiment.
The significant occurrence of vaginal cornification cell (p<0.05) after Linoleic acid supplementation indicated estrogenic activity of Linoleic acid which was in consistency with serum estradiol level, but this effect was not as much as CEE. Gama-Linolenic acid also exist a few cornified cell in smear which was not significantly differ from those control group.
Linoleic acid showed the beneficial effects on OVX rats' reproductive performance, thereby indicating its beneficial role in the treatment of the postmenopausal symptoms.
本研究旨在通过阴道角化试验评估黑种草籽的某些成分（包括亚油酸和γ-亚麻酸）可能的雌激素活性。
将40只16周龄的去卵巢（OVX）大鼠随机分为五组：阴性对照组（每天灌胃1 ml橄榄油）；阳性对照组（每天灌胃0.2 mg/kg共轭马雌激素-CEE）；实验组（分别每天灌胃50 mg/kg亚油酸、10 mg/kgγ-亚麻酸或15 mg/kg百里醌）。所有补充剂连续21天经胃内灌胃给药。为评估雌激素样活性，每天检查阴道涂片，并在基线、10天后和实验结束时测量血清雌二醇。
补充亚油酸后阴道角化细胞显著出现（p<0.05），表明亚油酸具有雌激素活性，这与血清雌二醇水平一致，但这种作用不如CEE明显。γ-亚麻酸涂片也存在少量角化细胞，与对照组无显著差异。
亚油酸对去卵巢大鼠的生殖性能有有益影响，从而表明其在治疗绝经后症状中的有益作用。
