Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, Turkey.
J Enzyme Inhib Med Chem. 2010 Feb;25(1):44-53. doi: 10.3109/14756360902932792.
In the present study, we determined the antioxidant activity of cepharanthine and fangchinoline from Stephania rotunda by performing different in vitro antioxidant assays, including 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, N,N- dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging, superoxide anion (O2*-) radical scavenging, hydrogen peroxide scavenging, total antioxidant activity, reducing power, and ferrous ion (Fe2+) chelating activities. Cepharanthine and fangchinoline showed 94.6 and 93.3% inhibition on lipid peroxidation of linoleic acid emulsion at 30 microg/mL concentration, respectively. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, and trolox indicated inhibitions of 83.3, 92.2, 72.4, and 81.3% on peroxidation of linoleic acid emulsion at the same concentration (30 microg/mL), respectively. According to the results, cepharanthine and fangchinoline have effective antioxidant and radical scavenging activity.
在本研究中，我们通过进行不同的体外抗氧化测定来确定头花千金藤碱和粉防己碱的抗氧化活性，包括 1,1-二苯基-2-苦基肼（DPPH）自由基清除、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除、N,N-二甲基-p-苯二胺二盐酸盐（DMPD）自由基清除、超氧阴离子（O2*-）自由基清除、过氧化氢清除、总抗氧化活性、还原能力和亚铁离子（Fe2+）螯合活性。头花千金藤碱和粉防己碱在 30μg/mL 浓度下分别对亚油酸乳液的脂质过氧化抑制率达到 94.6%和 93.3%。另一方面，丁基羟基茴香醚（BHA）、丁基羟基甲苯（BHT）、α-生育酚和 Trolox 在相同浓度（30μg/mL）下对亚油酸乳液的过氧化抑制率分别为 83.3%、92.2%、72.4%和 81.3%。根据结果，头花千金藤碱和粉防己碱具有有效的抗氧化和自由基清除活性。
