艰难梭菌感染诊断检测的评估。
Evaluation of diagnostic tests for Clostridium difficile infection.
机构信息
Department of Microbiology, City Hospital, Dudley Road, Birmingham, United Kingdom.
出版信息
J Clin Microbiol. 2010 Feb;48(2):606-8. doi: 10.1128/JCM.01579-09. Epub 2009 Dec 23.
Abstract
We evaluated toxigenic Clostridium difficile detection by a lateral flow assay for antigen and toxin, an enzyme immunoassay, and two commercial PCR methods. Compared to the cell cytotoxicity neutralization assay and toxigenic culture, both toxin detection methods lacked sensitivity. PCR following combined antigen and toxin detection provided the most useful diagnostic information.
摘要
我们评估了通过侧向流动检测抗原和毒素、酶免疫测定以及两种商业 PCR 方法检测产毒艰难梭菌的效果。与细胞细胞毒性中和测定和产毒培养相比，两种毒素检测方法均缺乏敏感性。联合抗原和毒素检测后进行 PCR 提供了最有用的诊断信息。
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